TY - JOUR AU - Loi, Vu Duc AU - Huong, Le Thi Thu AU - Tung, Bui Thanh AU - Xuan, Bui Thi PY - 2018/12/24 TI - Development of Functional Food Products and Skin Bleaching Cosmetics from Vietnamese Natural Sources JF - VNU Journal of Science: Medical and Pharmaceutical Sciences; Vol 34 No 2DO - 10.25073/2588-1132/vnumps.4124 KW - N2 - Abstract: Through data synthesis and insilico screening, 21 potential tyrosinase enzyme inhibiting compounds were identified. Specifically, Morus alba was identified as a herb containing tyrosinase enzyme inhibiting substances that could be used in skin whiteners. From Morus alba leaves, the following 11 flavonoid-terpenoid compounds were extracted and isolated: 3, 4-dihydroxybenzoic acid, ellagic acid, gallic acid, Kaempferol 3-O-β-D-glucopyranoside, Quercetin 3-O-α-L-rhamnopyranoside, isofraxidin-7-O-β-D-glucopyranoside, (±) -3, 5, 6, 7, 8, 4'-hexahydroxyflavane, Kaempferol-3, 7-O-α-L-rhamnopyranoside, 7, 4'-dihydroxy-5,3'-dimethoxyflavone, (S) -5, 5', 7-trihydroxy-2', and 4'-dimethoxy-6-methylflavanone. Acute toxicity, skin whitening effects on in vitro and in vivo with extracted fractions and isolated compounds were evaluated. The results show that the high safety fractions and compounds had good whitening effect. Based on the developed product recipe, pilot products in the form of capsule and cream were made and their skin whitening and skin irritant effects were evaluated. The results show that the two products have high safety, good skin whitening effect with no irritation. As a result, food supplement Morus capsules and MELAGENN cosmetics cream have been registered. Keywords: Morus alba, tyrosinase, insilico, flavonoid, terpenoid. References: [1] Đỗ Huy Bích, Đặng Quang Chung, Bùi Xuân Chương, Nguyễn Thượng Dong, Đỗ Trung Đàm, Phạm Văn Hiển, Vũ Ngọc Lộ, Phạm Duy Mai, Phạm Kim Mãn, Đoàn Thị Thu, Nguyễn Tập, Trần Toàn (2011), Cây thuốc và động vật làm thuốc ở Việt Nam, tập III,Nhà xuất bản Khoa học và kỹ thuật, Hà Nội, tr. 479-480. [2] Đỗ Tất Lợi (2005), Những cây thuốc và vị thuốc Việt Nam, Nxb Y học. [3] Viện Dược liệu (2004), Cây thuốc và động vật làm thuốc ở Việt Nam, NXB Khoa học kỹ thuật, tr.462-468. [4] Chang, L.-W., et al., (2011), Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food and Chemical Toxicology, 49(4): p. 785-790. [5] Du, Jiang, et al. (2003), "Antiviral flavonoids from the root bark of Morus alba L." Phytochemistry 62.8, 1235-1238. [6] A. Varnek, et al., (2008) ISIDA - Platform for Virtual Screening Based on Fragment and Pharmacophoric Descriptors. Current ComputerAided Drug Design 4 191. [7] Baurin, N., et al., (2002), Preliminary screening of some tropical plants for anti-tyrosinase activity. Journal of Ethnopharmacology, 2002. 82(2): p. 155-158. [8] Lan Jiang, Sodik Numonov, Khayrulla Bobakulov, Muhammad Nasimullah Qureshi Haiqing Zhao and Haji Akber Aisa, (2015), Phytochemical Profiling and Evaluation of Pharmacological Activities of Hypericum scabrum L., Molecules 20, 11257-11271 [9] Chunpeng Wan, Yanying Yu, Shouran Zhou, Shuge Tian and Shuwen Cao (2011), Isolation and identification of phenolic compounds from Gynura divaricata leaves, Pharmacogn Mag. 7(26): 101–108. [10] Viện Dược liệu (2006), Phương pháp nghiên cứu tác dụng dược lý của thuốc từ thảo dược, NXB Khoa học và kỹ thuật, tr. 139-141. [11] Granata, A., et al., (2007), Tyrosinase-generated quinones induce covalent modification, unfolding, and aggregation of human holo-myoglobin. Biomacromolecules, 8(10): p. 3214-3223. [12] Kim, Y.-J. and H. Uyama, (2005), Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cellular and Molecular Life Sciences, 62(15): p. 1707-1723. [13] Kubo, I., I. Kinst-Hori, and Y. Yokokawa, (1994), “Tyrosinase inhibitors from Anacardium occidentale fruits.” Journal of Natural Products, 57(4): p. 545-551. [14] Khan, S.B., et al., (2010), Tyrosinase inhibitory effect of benzoic acid derivatives and their structure-activity relationships. Journal of enzyme inhibition and medicinal chemistry, 25(6): p. 812-817. [15] Bộ Y tế, (2009), Dược điển Việt Nam 4.       UR - https://js.vnu.edu.vn/MPS/article/view/4124