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Abstract

Abstract: The heavy metal resistance and biosorption of two acid-tolerant yeast strains isolated from tea soils in Kagoshima Experimental Station (Japan) were investigated. Cryptococcus sp. AH-13 was more resistant to Cd, Cu, Zn, Co, Hg, Ag, Fe, Mn, Ni (except Pb) on the YG solid medium than Candida palmioleophila KB-6. The resistance to heavy metals in the YG solid medium were higher than those in the liquid medium. When being cultivated in YG liquid medium (pH 3.0) containing various concentrations of heavy metals, the growth of Candida palmioleophila KB-6 was considerably inhibited at 0.05 mM Cd, 0.3 mM Cu and 0.5 mM Zn whilst the growth of Cryptococcus sp. AH-13 in was inhibited at 0.5 mM Cd, 1.5 mM Cu and 1.5 mM Zn. Both types of living and dead cells of Candida palmioleophila KB-6 and Cryptococcus sp. AH-13 could remove heavy metals from their salt solutions. The amount of heavy metals accumulated in above types of yeast cells increases along with the concentration of heavy metals, but seems to be constant at a certain saturable concentration of heavy metals. Heavy metal biosorption by Cryptococcus sp. AH-13 appeared to be higher than that by Candida palmioleophila KB-6.

Keywords: Tea soil, acid-tolerant yeast, heavy metal resistant yeasts, Cryptococcus sp. AH-13, Candida palmioleophila KB-6.

References

[1] S. Kanazawa,T. Kunito, Preparation of pH 3.0 agar plate, enumeration of acid-tolerant and Al-resistant microorganisms in acid soils,Soil Sci. Plant Nutr., 42 (1996) 165.
[2] F. Kawai, D. Zhang, M. Sugimoto, Isolation and characterization of acid- and Al-tolerantmicroorganisms, FEMS Microbiol. Lett., 189 (2000) 143.
[3] S. Konishi, I. Souta, J. Takahashi, M. Ohmoto, S. Kaneko, Isolation and characteristics of acid- and aluminum-tolerant bacterium, Biosci. Biotech. Biochem., 58 (1994) 1960.
[4] A.I. Lopez-Archilla, I. Marin, R. Amils, Microbial ecology of an acidic river: biotechnological applications, In: Biohydrometallurgical, Processing II University of Chile, Santiago, 1995.
[5] V.A.T. Nguyen, K. Senoo, T. Mishima, M. Hisamatsu, Multiple Tolerance of Rhodotorula ghtirtis R-1 to Acid, Aluminum Ion and Manganese Ion, and Its Unusual Ability of Neutralizing Acidic Medium, J. Biosci. Bioeng., 92-4 (2001) 366.
[6] A. Rothstein, A.D. Hayes, The Relationship of the Cell Surface to Metabolism.XIII: The cation-binding properties of the yeast cell surface, Arch. Biochem. Biophys., 63 (1956) 87.
[7] K. Iwasaki, G. Yoshikawa, K. Sakurai, Fractionation of zinc in greenhouse soils, Soil Sci. Plant Nutr., 39 (1993) 507.
[8] J.A. Scott, S.J. Palmer, Sites of cadmium uptake in bacteria used for biosorption, Appl. Microbiol. Biotechnol., 33 (1990) 221.
[9] M.F. Abdullah, Comparative toxicity of heavy metals to some yeasts isolated from Saudi Arabian soil, Bioresour. Technol., 64 (1998) 193.
[10] G.M. Gadd, Interactions of Fungi with Toxic Metals, New Phytol.,124 (1993) 25.
[11] R.K. Poole, G.M. Gadd, Metals: Microbe Interactions, IRL Press, Oxford, 1989.
[12] C. White, G.M. Gadd, Determination of metals and metal fluxes in algae and fungi, Sci. Total Environ., 176 (1995) 107.
[13] K.J. Blackwell, I. Singleton, J.M. Tobin, Metal cation uptake by yeast: a review, Appl. Microbiol. Biotechnol., 43 (1995) 579.
[14] J. Tangaromsuk, P. Pokethitiyook, M. Kruatrachue, E.S. Upatham, Cadmium biosorption by Sphingomonas paucimobilis biomass, Bioresour. Technol., 85 (2002) 103.