The PCR Procedure to Detect Rapidly Two Common Bacterial Causing Pneumonia in Air Enviroment
Main Article Content
Abstract
In the environment, there are many bacteria, which can spread in the air, causing serious diseases like pneumonia, respiratory infections. Nowadays, culture is used as a traditional method for detecting and identifying bacteria. Although this method has highly sensitive, but it takes a long time to get results (24-48 hours). Therefore, the development of a rapid detection method and meanings to control air quality is essential. This study is designed to develop a procedure for the detection of bacterial causing pneumonia in the air with high specificity based on multiplex PCR. Acinetobacter baumannii and Pseudomonas aeruginosa were selected as research targets because they are two of the most dangerous bacteria, according to WHO.
Keywords:
Airborne infection, procedure, multiplex PCR, Acinetobacter baumannii, Pseudomonas aeruginosa.
References
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[2] C. Pasquarella, O. Pitzurra and A. Savino. The index of microbial air contamination. Journal of Hospital Infection 46, 2000, 241–256
[3] Daniela S, Lorna R, and Michael T, Advances in multiplex PCR: balancing primer efficiencies and improving detection success, Methods Ecol Evol 3(5), 2012, 898-905
[4] Vu D. P., Wertheim H. F. L., Larsson M., Nadjm B., Dinh Q. D., Nilsson L. E., Rydell U., Le T. D. T., Trinh H. S. , Pham H. M., Tran T. C., Doan T. H. H., Tran N. T., Le N. D., Huynh V. N., Tran P. T., Tran B. D., Nguyen T. S., Hanberger H. et al. Burden of Hospital Acquired Infections and Antimicrobial Use in Vietnamese Adult Intensive Care Units. Plos one tenth anniversary, 2016.
[5] Prevention of hospital-acquired infections, WHO, 2002.
[6] World Health Organization news release: http://www.who.int/mediacentre/news/releases/2017/bacteria-antibiotics-needed/en/
[7] Thong KL, Lai MY, Teh CSJ and Chua KH, Simultaneous detection of methicillin-resistant Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa by multiplex PCR, Tropical Biomedicine 28(1), 2011, 21–31.
[8] R Lavenir, D Jocktane, F Laurent, S Nazaret, B Cournoyer, Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target, Journal of Microbiological Methods 70, 2007, 20–29.
[9] Rzysztofik B. Microbiology of air. Wyd. Politechniki Warszawskiej, Warszawa, 1992.