Thien Van Le, Hieu Dang Trung Dam, Hong Thi Tham Le, Thao Phuong Nguyen, Chau Thi Tuong Ngo

Main Article Content

Abstract

Abstract: Biobed is a simple method to minimize point source contamination during manipulation of pesticides. The biomix is a principal element controlling the degradation efficacy of the biobed. The aim of this research was to evaluate the effect of inoculation with ligninolytic fungus Penicillium chrysogenum N2 on the biological activities and cartap degradation of biomix. Tests were made using biomixprepared with rice straw, spent mushroom substrate and top soil in a volumetric pro-portion of 2:1:1, moisture content of 60% and pre-incubation time of 15 days; cartap was added to the biomix at concentration of 100 mgkg-1. The results demonstrated that the ligninolytic enzyme activity in the inoculated biomix (645 U kg-1) was 8.5- fold higher compared with the non-inoculated one. Also, the higher microbial respiration (455 mg CO2100g-1) and counts of total aerobicbacteria(6.5´108 CFUg-1), actinomyces (1.3´108 CFUg-1), fungi (2.5´105 CFUg-1), cellulolytic microrganisms (4.68´105 CFUg-1), hemi-cellulolytic microrganisms (3.5´105CFU g-1) andligninolytic microrganisms (2.7´105 CFUg-1) were observed in the former. Besides, the Cartap degradation efficiency at 37oC for 10 days (99.42%) was higher in the inoculated biomix compared with the non-inoculated one (75.35%). In conclusion, it was recommended to use ligninolytic fungus Penicillium chrysogenum N2 as a inoculum to improve the biological activities and cartap degradation of biomix in biobed.


Keywords: Biobed, biomix, cartap degradation, ligninolytic fungus, Penicillium chrysogenum.


References


[1] N.H. Spliid, W. Brusch, O.S Jacobsen, S.U Hansen. In Pesticide point sources and dispersion of pesticides from a site previously used for handling of pesticides, 16th Danish Plant Protection Conference, Side effects of pesticides, Weeds, 1999; pp 33-46.
[2] M.D.P. Castillo, L. Torstensson, J. Stenström, Biobeds for Environmental Protection from Pesticide Uses- A Review, Journal of Agricultural and Food Chemistry 56 (2008) 6206.
[3] J. Husby, Biobeds in the world, 5th European Biobed Workshop, Throws Farm, UK, 2016.
[4] M.D.P Castillo, J. Stenström, P. Ander, Determination of manganese peroxidase activity with 3-methyl-2- benzothiazolinone hydrasone and 3-(dimethylamino) benzoic acid. Analytical Biochemistry 218 (1994) 399.
[5] W.H. Thompson, Test methods for the examination of composting and compost, U.S Composting Council and Department of Agriculture, U.S. Government Printing Office, Washington DC, 2002.
[6] S. Fernández-Alberti,O. Rubilar, G.R. Tortella, M.C. Diez1, Chlorpyrifos degradation in a Biomix: Effect of pre-incubation and water holding capacity, Journal of Soil Science and Plant Nutrition 12 (4) (2012) 785.
[7] M.D.P Castillo, L.Torstensson, Effect of biobed composition, moisture and temperature on the degradation of pesticides, Journal of Agricultural and Food Chemistry 55 (2007) 5725.


 

Keywords: Đệm sinh học, hỗn hợp sinh học, phân huỷ Cartap, nấm mốc phân huỷ lignin, Penicillium chrysogenum

References

[1] N.H. Spliid, W. Brusch, O.S Jacobsen, S.U Hansen. In Pesticide point sources and dispersion of pesticides from a site previously used for handling of pesticides, 16th Danish Plant Protection Conference, Side effects of pesticides, Weeds, 1999; pp 33-46.
[2] M.D.P. Castillo, L. Torstensson, J. Stenström, Biobeds for Environmental Protection from Pesticide Uses- A Review, Journal of Agricultural and Food Chemistry 56 (2008) 6206.
[3] J. Husby, Biobeds in the world, 5th European Biobed Workshop, Throws Farm, UK, 2016.
[4] M.D.P Castillo, J. Stenström, P. Ander, Determination of manganese peroxidase activity with 3-methyl-2- benzothiazolinone hydrasone and 3-(dimethylamino) benzoic acid. Analytical Biochemistry 218 (1994) 399.
[5] W.H. Thompson, Test methods for the examination of composting and compost, U.S Composting Council and Department of Agriculture, U.S. Government Printing Office, Washington DC, 2002.
[6] S. Fernández-Alberti,O. Rubilar, G.R. Tortella, M.C. Diez1, Chlorpyrifos degradation in a Biomix: Effect of pre-incubation and water holding capacity, Journal of Soil Science and Plant Nutrition 12 (4) (2012) 785.
[7] M.D.P Castillo, L.Torstensson, Effect of biobed composition, moisture and temperature on the degradation of pesticides, Journal of Agricultural and Food Chemistry 55 (2007) 5725.