Effect of Inoculation with Ligninolytic Fungus on the Biological Activities and Cartap Degradation of Biomix

Abstract

Abstract: Biobed is a simple method to minimize point source contamination during manipulation of pesticides. The biomix is a principal element controlling the degradation efficacy of the biobed. The aim of this research was to evaluate the effect of inoculation with ligninolytic fungus Penicillium chrysogenum N2 on the biological activities and cartap degradation of biomix. Tests were made using biomixprepared with rice straw, spent mushroom substrate and top soil in a volumetric pro-portion of 2:1:1, moisture content of 60% and pre-incubation time of 15 days; cartap was added to the biomix at concentration of 100 mgkg^-1. The results demonstrated that the ligninolytic enzyme activity in the inoculated biomix (645 U kg^-1) was 8.5- fold higher compared with the non-inoculated one. Also, the higher microbial respiration (455 mg CO₂100g^-1) and counts of total aerobicbacteria(6.5´10⁸ CFUg^-1), actinomyces (1.3´10⁸ CFUg^-1), fungi (2.5´10⁵ CFUg^-1), cellulolytic microrganisms (4.68´10⁵ CFUg^-1), hemi-cellulolytic microrganisms (3.5´10⁵CFU g^-1) andligninolytic microrganisms (2.7´10⁵ CFUg^-1) were observed in the former. Besides, the Cartap degradation efficiency at 37^oC for 10 days (99.42%) was higher in the inoculated biomix compared with the non-inoculated one (75.35%). In conclusion, it was recommended to use ligninolytic fungus Penicillium chrysogenum N2 as a inoculum to improve the biological activities and cartap degradation of biomix in biobed.

Keywords: Biobed, biomix, cartap degradation, ligninolytic fungus, Penicillium chrysogenum.

References

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