Ngo Thi Tuong Chau, Nguyen Thi Mai Luong, Phung Thi Ngoc Mai, Dao Van Huy, Le Van Thien

Main Article Content

Abstract

Abstract: From tea soils in Tan Cuong (Thai Nguyen, Vietnam), 16 acid- tolerant and aluminum- resistant fungi were isolated on Hansen plates (pH 3.0) with 100 mgL-1 Al. Two strains (F8 and F13) could grow wellon Hansen plates (pH 3.0) with 700 mgL-1 Al. Morphological and 28S rDNA sequence analyses indicated that strain F8 belonged to Eupenicillium javanicum, while strain F13 belonged to Penicillium variabile. Further investigation showed that both strains could grow actively in Hansen broth with 100 mgL-1 Al at pH 2.2- 5.0 and with 500- 2,000 mgL-1 Al at pH 2.4. After 7 days of incubation on a shaker at 30oC, 150 rpm, the aluminium absorption efficiency from Hansen broth with 2000 mgL-1 Al (pH 2.4) of strain F8 was 92.06% and that of strain F13 was 86.88%. The fungi areconsidered to be useful not only to improve acidified soils by decreasing the ionic aluminum concentration but also ensure quality of tea product.


Keywords: Acid soil, tea soil, acid-tolerant fungi, aluminum-resistant fungi, aluminium absorption.


References


[1] M. Hayatsu, Soil microflora and microbial activities in acid tea soils,Bull. Natl. Res. Veg. Ornam. Plants Tea B 6 (1993) 73 (in Japanese).
[2] Nioh, T. Isobe, M. Osada, Microbial biomass and some characteristics of a strongly acid tea field soil, Soil Sci. Plant Nutr. 39 (1993) 617-625.
[3] H Wang, R.K. Xu, N. Wang, X.H. Li, Soil acidification of Alfisols as influenced by tea cultivation in eastern China,Pedosphere 20(6) (2010) 799- 806.
[4] M.L. Jackson, P.M. Huang, Aluminum of acid soils in the food chain and senility, Sci. Total Environ. 28(1) (1983) 269-276.
[5] J. Edwardson, Aluminum and the pathogenesis of neurodegenerative disorders, Aluminium Food Environ. 2 (1988) 20-36.
[6] C.N. Martyn, Aluminium and Alzheimer's disease: an epidemiological approach, Environ Geochem. Health 12(1-2) (1990) 169-171.
[7] D. McLachlan, Aluminium and the risk for Alzheimer's disease, Environmetrics 6(3) (1995) 233-275.
[8] P. Illmer, K. Marschall, F. Schinner, Influence of available aluminum on soil-microorganisms, Lett. Appl. Microbiol. 21(1995)393-397.
[9] F. Kawai, D. Zhang, M. Sugimoto M, Isolation and charac- terization of acid-and Al-tolerant microorganisms. FEMS Microbiol. Lett. 189 (2000) 143-147.
[10] S. Kanazawa, N.T.T. Chau, S. Miyaki S, Identification and characterization of high acid tolerant and aluminum resistant yeasts isolated from tea soils,Soil Sci. Plant Nutr. 51 (2005) 671-674.
[11] N.T.T. Chau, L.V. Thien, S. Kanazawa, Identification and characterization of acidity-tolerant and aluminum-resistant bacterium isolated from tea soil, African Journal of Biotechnology 13(27) (2014) 2715-2726.
[12] G. He, X. Wang, G. Liao, S. Huang,J. Wu, Isolation, Identification and characterization of two aluminum- tolerant fungi from acidic red soil, Indian J. Microbiol. 56(3) (2016) 344-352.
[13] S.Kanazawa, T. Kunito T, Preparation of pH 3.0 agar plate, enumeration of acid-tolerant and Al-resistant microor- ganisms in acid soils, Soil Sci.Plant Nutr. 42 (1996) 165-173.
[14] A.K.N.Zoysa, A. Anandacoomaraswamy, M.S.D.L.De Silva, Management of soil Fertility in tea lands, Handbook on tea, Tea research institute of Sri Lanka, 2008; pp 27-33.

Keywords: Đất acid, đất trồng chè, nấm mốc chịu acid, nấm mốc kháng nhôm, hấp thụ nhôm

References

[1] M. Hayatsu, Soil microflora and microbial activities in acid tea soils,Bull. Natl. Res. Veg. Ornam. Plants Tea B 6 (1993) 73 (in Japanese).
[2] Nioh, T. Isobe, M. Osada, Microbial biomass and some characteristics of a strongly acid tea field soil, Soil Sci. Plant Nutr. 39 (1993) 617-625.
[3] H Wang, R.K. Xu, N. Wang, X.H. Li, Soil acidification of Alfisols as influenced by tea cultivation in eastern China,Pedosphere 20(6) (2010) 799- 806.
[4] M.L. Jackson, P.M. Huang, Aluminum of acid soils in the food chain and senility, Sci. Total Environ. 28(1) (1983) 269-276.
[5] J. Edwardson, Aluminum and the pathogenesis of neurodegenerative disorders, Aluminium Food Environ. 2 (1988) 20-36.
[6] C.N. Martyn, Aluminium and Alzheimer's disease: an epidemiological approach, Environ Geochem. Health 12(1-2) (1990) 169-171.
[7] D. McLachlan, Aluminium and the risk for Alzheimer's disease, Environmetrics 6(3) (1995) 233-275.
[8] P. Illmer, K. Marschall, F. Schinner, Influence of available aluminum on soil-microorganisms, Lett. Appl. Microbiol. 21(1995)393-397.
[9] F. Kawai, D. Zhang, M. Sugimoto M, Isolation and charac- terization of acid-and Al-tolerant microorganisms. FEMS Microbiol. Lett. 189 (2000) 143-147.
[10] S. Kanazawa, N.T.T. Chau, S. Miyaki S, Identification and characterization of high acid tolerant and aluminum resistant yeasts isolated from tea soils,Soil Sci. Plant Nutr. 51 (2005) 671-674.
[11] N.T.T. Chau, L.V. Thien, S. Kanazawa, Identification and characterization of acidity-tolerant and aluminum-resistant bacterium isolated from tea soil, African Journal of Biotechnology 13(27) (2014) 2715-2726.
[12] G. He, X. Wang, G. Liao, S. Huang,J. Wu, Isolation, Identification and characterization of two aluminum- tolerant fungi from acidic red soil, Indian J. Microbiol. 56(3) (2016) 344-352.
[13] S.Kanazawa, T. Kunito T, Preparation of pH 3.0 agar plate, enumeration of acid-tolerant and Al-resistant microor- ganisms in acid soils, Soil Sci.Plant Nutr. 42 (1996) 165-173.
[14] A.K.N.Zoysa, A. Anandacoomaraswamy, M.S.D.L.De Silva, Management of soil Fertility in tea lands, Handbook on tea, Tea research institute of Sri Lanka, 2008; pp 27-33.