Potential use of microbial product to degrade Chlorpyrifos in tea cultivation soil
Main Article Content
Abstract
Pesticides containing organophosphate (OP) compounds in general and Chlorpyrifos (CPF) in particular are used to control insects and pests. However, they have been proven to be highly toxic not only to some insects and wild animals but also have negative impacts on the environment and human health. This study provides an assessment of the effectiveness of using microbial product (MP) to degrade CPF in a large- and small-scale tea cultivation environment. The research results show that in some farming areas, residual CPF exists both in the soil and in agricultural products. When using the MP, the CPF content in tea decreased by 77.66%; the efficacy of CPF treatment in the soil reached 72-76% in the small scale . Additionally, the use of MP also increased the total microbial density in the net house scale from 106 to 107 CFU/g and beneficial microorganisms in the soil, stimulating growth and productivity for tea plants.
Keywords:
Microbial product; pesticides containing organophosphate, chlorpyrifos.
References
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[13] TCVN 9016:2011: Fresh Vegetables - Sampling Method In Production Fields (in Vietnamese).
[14] TCVN 7538-2:2005 (ISO 10381-2:2002): Soil Quality - Sampling - Part 2: Technical Guidelines For Sampling (in Vietnamese).
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[16] TCVN 6166 : 2002. Microbial Nitrogen Fixing Fertilizer (in Vietnamese).
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[19] M. S. Elshikh, K. M. Alarjani, D. S. Huessien, H. A. Elnahas, A. R. Esther, Enhanced Biodegradation of Chlorpyrifos by Bacillus cereus CP6 and Klebsiella pneumoniae CP19 from Municipal Waste Water, Environmental Research, Vol. 205, 2021, pp. 112438, https://doi.org/10.1016/j.envres.2021.112438.
[20] BS EN 15662:2008: Foods of Plant Origin - Determination of Pesticide Residues Using GC-MS and/or LC-MS/MS Following Acetonitrile Extraction/Partitioning and Clean-up By Dispersive SPE - QuEChERS-method, Europian Standard, 2008.
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[25] B.K. Singh, A. Walker, J.A. Morgan, D.J. Wright, Biodegradation of Chlorpyrifos by Enterobacter Strain B-14 and its Use in Bioremediation of Contaminated Soils, Applied and Environmental Microbiology, Vol. 70, No. 8, 2004,pp. 4855-4863, https://doi.org/10.1128/AEM.70.8.4855-4863.2004.
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[27] R. Bi, M. Ou, S. Zhou, S. Geng, Y. Zheng, J. Chen, R. Mo , Y. Li, G. Xiao, X. Chen, S. Zhai, A. Zhang, B. Fang, Degradation Strategies of Pesticide Residue: from Chemicals to Synthetic Biology, Synthetic and Systems Biotechnology, Vol. 8, No. 2, 2023, pp. 302-313, https://doi.org/10.1016/j.synbio.2023.03.005.
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[29] J. Abraham, S. Silambarasan, Biodegradation of Chlorpyrifos and its Hydrolysis Product 3,5,6-trichloro-2-pyridinol Using a Novel Bacterium Ochrobactrum sp. JAS2: A proposal of its Metabolic Pathway, Pesticide Biochemistry and Physiology, Vol. 126, 2016, pp. 13-21, https://doi.org/10.1016/j.pestbp.2015.07.001.
[30] A. C. Gonçalves, E. C. Junior, D. Schwantes,
A. Pinheiro, V. Kaufmann, A. Snak, Environmental Fate of Chlorpyrifos in Rhodic Ferralsol Grown with Corn during Summer and Winter Seasons under High-intensity Rainfall, Groundwater for Sustainable Development,
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[31] J. Abraham, S. Silambarasan, Biodegradation of Chlorpyrifos and Its Hydrolysis Product 3,5,6-trichloro-2-pyridinol Using a Novel Bacterium Ochrobactrum sp. JAS2: A Proposal of Its Metabolic Pathway, Pesticide Biochemistry and Physiology, Vol. 126, 2016, pp. 13-21, https://doi.org/10.1016/j.pestbp.2015.07.001.
[32] N. T. Lam, N. K. B. Tam, L. D. Trung, P. H. Thuong, D. V. Duong, T. Thanh, Enhancing Soil Bioremediation: Microbial Composting Strategies for the Degradation of Chlorpyrifos Ethyl in Agricultural Soils, E3S Web of Conferences, Vol. 559, 2024, pp. 04026, https://doi.org/10.1051/e3sconf/202455904026.
[2] S. Chen, C. Liu, C. Peng, H. Liu, M. Hu, G. Zhong, Biodegradation of Chlorpyrifos and Its Hydrolysis Product 3,5,6-Trichloro-2-Pyridinol by a New Fungal Strain Cladosporium cladosporioides Hu-01, PLoS One, Vol. 7, No 10, 2012, pp. 47205, https://doi.org/10.1371/journal.pone.0047205.
[3] C. Oltramare, F. Weiss, P. Staudacher, O. Kibirango, A. Atuhaire, C. Stamm, Pesticides Monitoring in Surface Water of a Subsistence Agricultural Catchment in Uganda Using Passive Samplers, Environmental Science and Pollution Research, Vol. 30, 2022, pp. 10312-10328, https://doi.org/10.1007/s11356-022- 22717-2.
[4] F. K. Sishu, S. A. Tilahun, P. Schmitter, G. Assefa, T. S. Steenhuis, Pesticide Contamination of Surface and Groundwater in an Ethiopian Highlands' Watershed. Water, Vol. 14, No. 21, 2022, pp. 3446, https://doi.org/10.3390/w14213446.
[5] T. Takayasu, H. Yamamoto, Y. Ishida, M. Nosaka, M. Kawaguchi, Y. Kuninaka, A. Kimura, T. Kondo, Postmortem Distribution of Chlorpyrifos-methyl, Fenitrothion and Their Metabolites in Body Fluids and Organ Tissues of an Intoxication Case, Legal Medicine, Vol. 29, 2017, pp. 44-50, https://doi.org/10.1016/j.legalmed.2017.10.002.
[6] T. T. N. Hang, Research on The Production and Application of Microbial Preparations for the Decomposition of Organic Phosphorus (OP) Contributing to Reducing Environmental Pollution and Increasing Crop Productivity, Final Report of The Research Project Under the National Science and Technology Program: Agricultural and Aquaculture Biotechnology, 2020 (in Vietnamese).
[7] E.Wołejko, B. Łozowicka, A. J. Trypuć,
M. Pietruszyńska, U. Wydro, Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review, International Journal of Environmental Research and Public Health, Vol. 26, No. 19, 2022, pp. 12209, https://doi.org/10.3390/ijerph191912209.
[8] Y. Huang, W. Zhang, S. Pang, J. Chen, P. Bhatt, S. Mishra, S. Chen, Insights into The Microbial Degradation and Catalytic Mechanisms of Chlorpyrifos, Environmetal Research, Vol. 194, 2021, pp. 110660, https://doi.org/10.1016/j.envres.2020.110660.
[9] S. Bose, P. S. Kumar, D. V. N. Vo, A Review on the Microbial Degradation of Chlorpyrifos and Its Metabolite TCP, Chemosphere, Vol. 283, 2021, pp. 131447, https://doi.org/10.1016/j.chemosphere.2021.131447.
[10] S. Jaiswal, J. K. Bara, R. Soni, K. Shrivastava, Bioremediation of Chlorpyrifos Contaminated Soil by Microorganism, International Journal of Environment Agriculture and Biotechnology,
Vol. 2 , No. 4, 2017, pp. 1624-1630, https://doi.org/10.22161/ijeab/2.4.21.
[11] S. Bose, P. S. Kumar, N. V. D. Viet, A Review on the Microbial Degradation of Chlorpyrifos and its Metabolite TCP, Chemosphere, Vol. 283, 2021, pp. 131447, https://doi.org/10.1016/j.chemosphere.2021.131447.
[12] QCVN 01-28:2010/BNNPTNT: National Technical Regulation on Tea - Sampling Process for Analyzing Quality, Safety, and Food Hygiene (in Vietnamese).
[13] TCVN 9016:2011: Fresh Vegetables - Sampling Method In Production Fields (in Vietnamese).
[14] TCVN 7538-2:2005 (ISO 10381-2:2002): Soil Quality - Sampling - Part 2: Technical Guidelines For Sampling (in Vietnamese).
[15] TCVN 4884-1:2015 (ISO 4833-1:2013): Microorganism Quantification Method (in Vietnamese).
[16] TCVN 6166 : 2002. Microbial Nitrogen Fixing Fertilizer (in Vietnamese).
[17] J. Gan, S. K. Papiernik, W. C. Koskinen, S. R. Yates, Evaluation of Accelerated Solvent Extraction (ASE) for Analysis of Pesticide Residues in Soil, Environmental Science and Technology, Vol. 33, No. 18, 1999, pp. 3249-3253, https://doi.org/10.1021/es990145+.
[18] Y. Gao, S. Chen, M. Hu, Q. Hu, J. Luo, Y. Li Purification and Characterization of a Novel Chlorpyrifos Hydrolase from Cladosporium cladosporioides Hu-01, PLoS ONE, Vol. 7, No. 6, 2012, pp. 38137, https://doi.org/10.1371/journal.pone.0038137.
[19] M. S. Elshikh, K. M. Alarjani, D. S. Huessien, H. A. Elnahas, A. R. Esther, Enhanced Biodegradation of Chlorpyrifos by Bacillus cereus CP6 and Klebsiella pneumoniae CP19 from Municipal Waste Water, Environmental Research, Vol. 205, 2021, pp. 112438, https://doi.org/10.1016/j.envres.2021.112438.
[20] BS EN 15662:2008: Foods of Plant Origin - Determination of Pesticide Residues Using GC-MS and/or LC-MS/MS Following Acetonitrile Extraction/Partitioning and Clean-up By Dispersive SPE - QuEChERS-method, Europian Standard, 2008.
[21] H Mali, C. Shah, D. H. Patel, U. Trivedi, R. B. Subramanian, Degradation Insight of Organophosphate Pesticide Chlorpyrifos Through Novel Intermediate 2,6-dihydroxypyridine by Arthrobacter sp. HM01, Bioresources and Bioprocessing, Vol. 9, No. 1, 2022, pp. 31, https://doi.org/10.1186/s40643-022-00515-5.
[22] QCVN 01-189:2019/BNNPTNT: National Technical Regulation on Fertilizer Quality (in Vietnamese).
[23] R. A. Gilani, M. Rafique, A. Rehman, M. F. Munis, S. Y. Rehman, H. J. Chaudhary, Biodegradation of Chlorpyrifos by Bacterial Genus Pseudomonas. Journal of Basic Microbiology, Vol. 56, 2016,
pp. 105-119, https://doi.org/10.1002/jobm.201500336.
[24] M. Farhan, M. Ahmad, A. Kanwal, Z. A. Butt, Q. F. Khan, S. A. Raza, H. Qayyum, A. Wahid, Biodegradation of Chlorpyrifos Using Isolates from Contaminated Agricultural Soil, its Kinetic Studies, Scientific Report, Vol. 11, No. 1, 2021, pp. 10320, https://doi.org/10.1038/s41598-021-88264-x.
[25] B.K. Singh, A. Walker, J.A. Morgan, D.J. Wright, Biodegradation of Chlorpyrifos by Enterobacter Strain B-14 and its Use in Bioremediation of Contaminated Soils, Applied and Environmental Microbiology, Vol. 70, No. 8, 2004,pp. 4855-4863, https://doi.org/10.1128/AEM.70.8.4855-4863.2004.
[26] A. Mehta, K. Bhardwaj, M. Shaiza, R. Gupta, Isolation, Characterization and Identification of Pesticide Degrading Bacteria from Contaminated Soil for Bioremediation, Biologia Futura, Vol. 72, No. 3, 2021, pp. 317-323, https://doi.org/10.1007/s42977-021-00080-6.
[27] R. Bi, M. Ou, S. Zhou, S. Geng, Y. Zheng, J. Chen, R. Mo , Y. Li, G. Xiao, X. Chen, S. Zhai, A. Zhang, B. Fang, Degradation Strategies of Pesticide Residue: from Chemicals to Synthetic Biology, Synthetic and Systems Biotechnology, Vol. 8, No. 2, 2023, pp. 302-313, https://doi.org/10.1016/j.synbio.2023.03.005.
[28] N. Islam, R. Iyer, Functional Analysis of Chlorpyrifos Biodegradation in Agricultural Soils Augmented with a Three-Strain Bacterial Consortium, Water, Air, & Soil Pollution,
Vol. 232, No. 425, 2021, https://doi.org/10.1007/s11270-021-05349-z.
[29] J. Abraham, S. Silambarasan, Biodegradation of Chlorpyrifos and its Hydrolysis Product 3,5,6-trichloro-2-pyridinol Using a Novel Bacterium Ochrobactrum sp. JAS2: A proposal of its Metabolic Pathway, Pesticide Biochemistry and Physiology, Vol. 126, 2016, pp. 13-21, https://doi.org/10.1016/j.pestbp.2015.07.001.
[30] A. C. Gonçalves, E. C. Junior, D. Schwantes,
A. Pinheiro, V. Kaufmann, A. Snak, Environmental Fate of Chlorpyrifos in Rhodic Ferralsol Grown with Corn during Summer and Winter Seasons under High-intensity Rainfall, Groundwater for Sustainable Development,
Vol. 23, 2023, pp. 100985, https://doi.org/10.1016/j.gsd.2023.100985.
[31] J. Abraham, S. Silambarasan, Biodegradation of Chlorpyrifos and Its Hydrolysis Product 3,5,6-trichloro-2-pyridinol Using a Novel Bacterium Ochrobactrum sp. JAS2: A Proposal of Its Metabolic Pathway, Pesticide Biochemistry and Physiology, Vol. 126, 2016, pp. 13-21, https://doi.org/10.1016/j.pestbp.2015.07.001.
[32] N. T. Lam, N. K. B. Tam, L. D. Trung, P. H. Thuong, D. V. Duong, T. Thanh, Enhancing Soil Bioremediation: Microbial Composting Strategies for the Degradation of Chlorpyrifos Ethyl in Agricultural Soils, E3S Web of Conferences, Vol. 559, 2024, pp. 04026, https://doi.org/10.1051/e3sconf/202455904026.