Characterization of Crilin and Nanocurcumin’s Synergistic Effect on Treatment for 7.12-Dimethylbenz[a]anthracene (DMBA)-Induced Breast Cancer Mice

Abstract

Breast cancer is the neoplastic disease which is characterized by unregulated ductal and lobular hyperplasia. Some herbal remedies have proved the inhibitory effect on breast cancer, such as crilin-extracted from Cirnum latifolum and curcumin-isolated from Cucuma longa. However, the synergistic effect of crilin and nanocurcumin has not been studied so far. In this study, we established the mouse model of breast cancer induced by DMBA and evaluated the effectiveness of the combination of crilin and nanocurcumin in treatment of breast cancer. After a 12-week co-administration of crilin and nanocurcumin, the DMBA-induced mice’s body weight and the number of erythrocytes and leukocytes in their blood reversed. Furthermore, the synergistic effect of crilin and nanocucumin on reduction in the tumor volume was proven. Histological analysis revealed that co-administration of crilin and nanocurcumin inhibited the expansion of mammary ductal carcinoma cells into surrounding tissues, recovered lobular cells structure, and diminished leukocyte composition. Thereby, the combination of crilin and nanocurcumin helped recover immune system and prevent further development of breast cancer.

Keywords

Breast cancer, DMBA, Cirnum latifolum, nanocucumin, synergistic effect.

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