BK Polyomavirus Genotypes in Renal Transplant Recipients in Northern Vietnam

Abstract

BK polyomavirus (BKV), a ubiquitous opportunistic infection among humans, causes BKV-associated nephropathy (BKVN) after renal transplantation. BKV strains worldwide are classified into 4 genotypes (I-IV), among which genotype I and IV are subdivided into 4 and 6 subtypes, respectively. Based on 580 bp-long VP1 sequences, this study analyzed the genotypes of 6 BKV strains from 20 specimens including plasma and urine from renal allograft recipients in Northern Vietnam. The VP1 genes of the BKV strains were analyzed by BLAST (NCBI), Bioedit, combined with MEGA7.0 software to construct and analyze the phylogenetic tree. Several mutations (8-39/ 580 nucleotides) were identified in all the 6 BKV strains compared to reference Dunlop BKV strain, among which BKV1 strain’s subtype IVc1 (isolated from the BKVN patients) had the most mutation nucleotides (39/ 580). The remaining 5 strains of BKV belonged to genotype I. The study provides a valuable fundamental molecular database contributing to monitoring as well as screening strategies and treatment options for BKV in kidney allograft recipients in Vietnam.

 Keywords:

BK polyomavirus, BKV-associated nephropathy (BKVN), renal transplantation, genotype.

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