Establishing the Protocol for Detecting rs11212617 Polymorphism Related to Metformin Response in Type 2 Diabetic Patients

Abstract

Metformin is currently recommended as a first-line drug for treatment of hyperglycemia in patients with type 2 diabetes. Nevertheless, the drug’s treatment effect is not uniform in all patients. The recent studies have indicated that the single-nucleotide polymorphism (SNP) rs11212617 (C>A) (11q22.3), located near the ataxia telangiectasia mutated (ATM) gene, is associated with Metformin treatment response, in which, allele C is better than allele A. Therefore, an SNP detecting protocol for a group of Vietnamese type 2 diabetic patients was to be established. The DNA, PCR and sequencing-extracted blood samples were applied to identify rs11212617 polymorphism among 22 Vietnamese patients. Out of the 22 patients genotyped for rs11212617 polymorphism, CC and AA were the homozygous genotypes with frequency of 54.5% and 13.7%, respectively. 31.8% of the patients had heterozygous genotype CA. The frequencies of allele C and allele A were 0.71 and 0.29, respectively. This result is expected to help develop further studies aimed to evaluate the association between genetic polymorphism with clinically drug response in Vietnamese population and thereby, improving the effectiveness of treatment.

Keywords

Single nucleotide polymorphism, rs11212617, ATM gene, metformin, type 2 diabetes.

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