Associations of Single Nucleotide Polymorphism rs17782313 in Melanocortin 4 Receptor Gene with Anthropometric Indices in Normal and Obesity Primary School Children in Hanoi

Abstract

Abstract: Melanocortin-4 receptor (MC4R) plays an important role in regulating food intake and energy balance. A number of studies have revealed that variant rs17782313 is significantly associated with obesity in different populations. However, its role to obesity in the Vietnamese populations has not been identified. This study aims to evaluate the association of rs17782313 with anthropometric indices and obesity in a group of Vietnamese children. A case-control study was conducted on 559 children aged 6-11 years (278 obese cases and 281 normal controls). The nutrition status of the children was classified using both the criteria of the International Obesity Task Force 2000 and those of the World Health Organization 2007. The results show that in the normal group, the data of z score of weight for age was the highest in CC genotype group and was the lowest in TT genotype group (0.11 and -0.38, respectively, p=0.023). In the obese group, the waist hip ratio was the lowest in TT genotype group and the highest one was in CC genotype group (0.93 and 0.97 respectively, p=0.031). The study findings indicate that variant rs17782313 of MC4R is likely to have an impact on the changing of weight and central obesity in the Vietnamese children.

Keywords: rs17782313, MC4R, obesity, anthropometric indices, Vietnamese children.

References

[1] C.H. Andreasen, G. Andersen, Gene - environment interactions and obesity - Further aspects of genome wide association studies, Nutrition 25(10), (2009), 998.

[2] T. Fall, E. Ingelsson, Genome–wide association studies of obesity and metabolic syndrome, Mol Cell Endocrinol, 382(1), (2012), 740.

[3] Y. Lu, R.J. Loos, Obesity genomics: assessing the transferability of susceptibility loci across diverse populations, Genome Med, 5(6), (2013), 55.

[4] C. Cyrus, M.H. Ismail, S. Chathoth, et al., Analysis of the impact of common polymorphisms of the FTO and MC4R Genes with the risk of severe obesity in Saudi Arabian population, Genet Test Mol Biomarkers, 22(3), (2018), 170.

[5] A.M. de Carvalho, P. Shao, H. Liu et al., The MC4R genotype is associated with postpartum weight reduction and glycemic changes among women with prior gestational diabetes: longitudinal analysis. Sci Rep 7(1), (2017), 9654.

[6] N. Balthasar, L.T. Dalgaard, C.E. Lee et al., Divergence of melanocortin pathways in the control of food intake and energy expenditure. Cell, 123(3), (2005), 493.

[7] R.D. Cone, Anatomy and regulation of the central melanocortin system, Nat Neurosci 8(5), (2005), 571.

[8] J.F. Davis, D.L. Choi, J.D. Shurdak et al., Central melanocortins modulate mesocorticolimbic activity and food seeking behavior in the rat. Physiol Behav 102(5), (2011), 491.

[9] V. Turcot, Y. Lu, H.M. Highland et al., Publisher Correction: Protein-altering variants associated with body mass index implicate pathways that control energy intake and expenditure in obesity. Nat Genet (2018).

[10] C.M.M. Resende, D.F. Durso, K.B.G. Borges et al., The polymorphism rs17782313 near MC4R gene is related with anthropometric changes in women submitted to bariatric surgery over 60 months. Clin Nutr. 5614(17), (2017), 30179-6.

[11] A.L. Hasselbalch, L. Angquist, L. Christiansen et al., A variant in the fat mass and obesity-associated gene (FTO) and variants near the melanocortin-4 receptor gene (MC4R) do not influence dietary intake, J Nutr. 140(4), (2010), 831.

[12] S.F. Grant, J. P. Bradfield, H. Zhang H, et al., Investigation of the locus near MC4R with childhood obesity in Americans of European and African ancestry. Obesity (Silver Spring). 17(7), (2009), 1461.

[13] L.T. Tuyet, B.T. Nhung, D.T.A. Dao, et al., The brain-derived neurotrophic factor val66met polymorphism, delivery method, birth weight, and night sleep duration as determinants of obesity in Vietnamese children of primary school age, Childhood Obesity, (2017), DOI: 10.1089/chi.2017.0007.

[14] Lê Thị Tuyết, Trần Quang Bình, Bước đầu nghiên cứu đa hình nucleotide đơn MC4R-rs17782313 ở trẻ 5-6 tuổi Hà Nội bằng phương pháp PCR-RFLP, Tạp chí Khoa học Đại học Quốc gia Hà Nội, chuyên san KHTN và Công nghệ, 31 (3), (2015), 57.

[15] R.J. Loos, C.M. Lindgren, S. Li, et al., Common variants near MC4R are associated with fat mass, weight and risk of obesity, Nat Genet. 40(6), (2008), 768.

[16] W. Huang, Y. Sun, J. Sun, et al., Combined effects of FTO rs9939609 and MC4R rs17782313 on obesity and BMI in Chinese Han populations, Endocrine, 39(1), (2011), 69.

[17] D. Lv, D.D. Zhang, H. Wang, et al., Genetic variations in SEC16B, MC4R, MAP2K5 and KCTD15 were associated with childhood obesity and interacted with dietary behaviors in Chinese school-age population, Gene, 560(2), 2015, 149.

[18] L. Tao, Z. Zhang, Z. Chen, et al., A Common variant near the melanocortin 4 receptor is associated with low-density lipoprotein cholesterol and total cholesterol in the Chinese Han population, Mol Biol Rep, 39(6), (2012), 6487.

[19] J. Wang, H. Mei, W. Chen et al., Study of eight GWAS-identified common variants for association with obesity-related indices in Chinese children at puberty, Int J Obes (Lond) 36(4), (2012), 542.