The Association of Single Nucleotide Polymorphism Rs6548238 in TMEM18 Gene with Obesity in Hanoi Primary School Children

Abstract

The transmembrane 18 gene (TMEM18) has been found associating with obesity risk in European, Chinese, and Japanese descents. However, the contribution of TMEM18 gene to obesity in the Vietnamese population has not been fully described. The purpose of the present study is to evaluate the association of rs6548238 TMEM18 gene with obesity in Hanoi primary school children. A case-control study was conducted on 559 children aged 6-11 (278 obese cases and 281 normal controls). The nutrition status of the children was classified using both the criteria of International Obesity Task Force 2000 and World Health Organization 2007. The results showed that the prevalence of risk allele (allele C) was higher in obese group than that in normal group (0.964 vs. 0.939, P=0.056). After adjusting for age, sex, there was an association between SNP rs6548238 and obesity in the children of the recessive model (the children with CC genotype had 1.8 times higher risk of obesity than the children with TT and CT genotypes) and of the additive (per allele C) model (one allele C increased the risk of obesity by 1.7 times).

Keywords

Obesity, children, TMEM18 gene, rs6548238.

References

[1] C.B. Ebbeling, D.B. Pawlak, D.S. Ludwig, Childhood obesity: public-health crisis, common sense cure, Lancet, 360(9331), (2002), 473.
[2] Viện dinh dưỡng quốc gia và Quỹ nhi đồng Liên hiệp quốc (Unicef), Báo cáo Tổng điều tra dinh dưỡng 2009 - 2010, (2012), 44.
[3] Bùi Thị Nhung, Lê Thị Hợp, Trần Quang Bình và cs, Tình trạng dinh dưỡng của học sinh tiểu học nội thành Hà Nội năm 2011, Tạp chí Y học Dự phòng, XXIII, 1(136), (2013), 49.
[4] G. Taubes, As obesity rates rise, experts struggle to explain why, Science, 280(5368), (1998), 1367.
[5] M. Melania, D. Bruno, Genetics of Pediatric Obesity. Pediatrics, (2012), doi: 10.1542/peds.2011-2717.
[6] M.S. Ellulu, M.O. Jalambo, Gene-environment Interaction: The Causes of High Obesity Incidence, Kathmandu Univ Med J (KUMJ), 15(57), (2017), 91.
[7] T. Fall, E. Ingelsson, Genome-wide association studies of obesity and metabolic syndrome, Mol Cell Endocrinol, 382(1), (2014), 740.
[8] K.L. Monda, G.K. Chen, K.C. Taylor, et al., A meta-analysis identifies new loci associated with body mass index in individuals of African ancestry, Nat Genet, 45(6), (2013), 690.
[9] A.C. Locke, B. Kahali, S.I. Berndt, et al., Genetic studies of body mass index yield new insights for obesity biology, Nature, 518(7538) (2015), 197.
[10] M.S. Almén, J.A. Jacobsson, J.H. Shaik, et al., The obesity gene, TMEM18, is of ancient origin, found in majority of neuronal cells in all major brain regions and associated with obesity in severely obese children, BMC Med Genet, 11, (2010), 58.
[11] M. Rask-Andersen, J.A. Jacobsson, G. Moschonis, et al., Association of TMEM18 variants with BMI and waist circumference in children and correlation of mRNA expression in the PFC with body weight in rats, Eur J Hum Genet, 20(2), (2012), 192.
[12] J. Zhao, J.P. Bradfield, M. Li, et al., The role of obesity-associated loci identified in genome-wide association studies in the determination of pediatric BMI, Obesity (Silver Spring), 17(12), (2009), 2254
[13] Le Thi Tuyet, Tran Quang Binh, Duong Thi Anh Dao và cs., Application of restriction fragment leghth polymorphirm method for genotyping TMEM18 rs6548238 polymorphism, Journal of biology, 37(1se), (2015), 85.
[14] Lê Thị Tuyết, Dương Thị Anh Đào, Tính đa hình và đặc điểm nhân trắc ở các nhóm kiểu gen của đa hình nucleotide đơn rs6548238 gen TMEM18 ở trẻ tiểu học Miền Bắc, Việt Nam, Tạp chí Khoa học ĐHQGHN: Khoa học Tự nhiên và Công nghệ, 33(1), (2017), 95.
[15] Bui Thi Nhung, Tran Quang Binh, Malnutrition primary school children in a suburban district of Hanoi, 2011. Vietnam medical asociation, XXV, 2(162), (2015), 30.
[16] WHO (2007), Growth reference 5-19 years, http://www.who.int/growthref/who2007_bmi_for_age/en/index.html.
[17] K. Hotta, M. Nakamura, T. Nakamura, et al., Association between obesity and polymorphisms in SEC16B, TMEM18, GNPDA2, BDNF, FAIM2 and MC4R in a Japanese population, J Hum Genet, 54(12), (2009), 727.
[18] J. Wang, H. Mei, W. Chen, et al., Study of eight GWAS-identified common variants for association with obesity-related indices in Chinese children at puberty, Int J Obes (Lond), 36(4), (2012), 542.
[19] M. Rask-Andersen, J.A. Jacobsson, G. Moschonis, et al., Association of TMEM18 variants with BMI and waist circumference in children and correlation of mRNA expression in the PFC with body weight in rats. Eur J Hum Genet, 20(2), (2012), 192.
[20] A. Hinney, C. Vogel, J. Hebebrand, From monogenic to polygenic obesity: recent advances, Eur Child Adolesc Psychiatry, 19(3) (2010), 297.
[21] W.H. Dietz, Health consequences of obesity in youth: childhood predictors of adult disease, Pediatrics, 101(3), (1998), 51.