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Abstract: This study develops a high-performance liquid chromatographic method with fluorecence detection for the quantification of flurbiprofen in tablets. A C8 reverse phase column (5 μm, 120 Å, 4.6×150 mm) was used with a mobile phase of acetonitrile: water: glacial acetic acid (65 : 32.5 : 2.5, v/v/v). The flow rate was 1 ml/min and the column effluent was monitored within 8 minutes. The excitation wave length and emission wave length were 247 nm and 312 nm, respectively. The temperature of flow cell was set at 45˚C and detector sensitivity was 5. The retention time of flurbiprofen was found to be 3.78 minutes. The method was validated under the concentration range of 5 - 100 ng/ml with a tight linear correlation between peak area and sample concentration (R = 0.9999). The proposed method was found to be specific to flurbiprofen, accurate and precise. The percentage recovery was ≤ 100 ± 2%, the relative standard deviation of the repeatability was ≤ 2.08% and the relative standard deviation of the intermediate precision was ≤ 2.96%. The limit of detection and limit of quantification were 0.010 and 0.025 ng/ml, respectively.
Keywords: Flurbiprofen, quantification, high-performance liquid chromatography, fluorecence detection.
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