Development and Validation of a HPLC Method of Quantification of Caffeine and EGCG in Green Tea (Camellia sinensis L.) Extract

Abstract

This study investigates a simple high performance liquid chromatography (HPLC) in an isocratic eluent manner for quantification of caffeine and EGCG in green tea extract. The study separation system contained a C18-RP column (250 × 4.6 mm, 5 µm), a mobile phase of methanol/phosphoric acid/water (20/0.1/79.9, v/v) and a DAD detector at 280 nm. This system proves convenient for rapid routine analysis of caffeine and EGCG in green tea extract with good repeatability and accuracy.

Keywords

HPLC, caffeine, EGCG, green tea, extract.

References

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