Evaluating the Antioxidant and Xanthine Oxidase Enzyme Inhibitory Activities in vitro of Piper betle Linn. Leaf Extract

Abstract

In this study, leaves of Piper betle L. were extracted by ultrasonic with ethanol 50% and successively fractionated with n-hexane, ethyl acetate (EtOAc) and n-butanol (n-BuOH) solvents. These fractions were evaluated for their antioxidant and xanthine oxidase inhibitory activities in vitro. The study results show that EtOAc fraction had the highest antioxidant effect (IC₅₀: 15.54 ± 0.48 µg/mL), followed by EtOH fraction (IC₅₀: 31.31 ± 0.12 µg/mL), n-hexane fraction (IC₅₀: 83.67 ± 0.14 µg/mL), and the lowest was n-BuOH fraction (IC₅₀: 95.60 ± 0.37 µg/mL). The evaluation of XO enzyme inhibition shows that EtOAc fraction extract had the strongest XO enzyme inhibitory activity (IC₅₀: 29.65 ± 0.93 µg/mL), followed by n-BuOH fraction (IC₅₀: 37.22 ± 1.23 µg/mL), EtOH fraction (IC₅₀: 52.13 ± 0.56 µg/mL), and the lowest was n-hexane fraction (IC₅₀: 80.12 ± 0.21 µg/mL). These results indicate that the EtOAc fraction from the leaf of Piper betle L. can be used for the prevention and treatment of gout.

Keywords

Gout, Piper betle Linn., xanthine oxidase, antioxidant activity

References

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