Evaluation of Antioxidant and α-glucosidase Inhibitory Activities of Codonopsisjavanica (Blume) Hook. f. Thoms’ Root Extract

Abstract

This study aims to evaluate the antioxidant ability and α-glucosidase inhibitory activities of Codonopsisjavanica extract to elucidate its mechanism in the treatment of diabetes type 2. The roots of Codonopsisjavanica were extracted with ethanol solvents and fractionated with n-hexane, ethyl acetate and butanol solvents. The total extract and the fractions were evaluated for free radical scavenging by 2.2-diphenyl-1-picrylhydrazyl method and α-glucosidase inhibitory activity in vitro. The study results show that ethyl acetate fraction from Codonopsisjavanica roots had the strongest antioxidant activity with a value of IC₅₀ of 80.6 ± 2.8 µg/mL and a strong α-glucosidase enzyme inhibitory activity with a value of IC₅₀ of 80.4 ± 5 µg/mL. These data suggest that ethyl acetate fraction from Codonopsisjavanica roots may have potential for the prevention and treatment of diabetes type 2.

Keywords

Codonopsisjavanica, diabetes type 2, α-glucosidase, antioxidant ability, fraction.

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