Effects of Vitamin E on FasL-induced Apoptotic Death of Dendritic Cells through PI3K Signaling

Abstract

This study addresses the effects of vitamin E (VitE) on the survival of mouse dendritic cells (DCs) and on underlying signaling molecules. To this end, mouse bone marrow cells were isolated and cultured to attain bone marrow-derived DCs (BMDCs). The cells were treated with FasL in the presence or absence of VitE. Western blotting and FACS analysis was performed to determine the expression of signaling molecules and their involvement in DC apoptosis. The FasL treatment resulted in activation of caspase 8 and an increased number of Annexin V⁺ cells; the effects were significantly suppressed when VitE was present in the cell culture. Importantly, the anti-apoptotic effects of VitE were abolished by using pharmacological inhibition of PI3K signaling with LY294002. The study results show that VitE inhibited FasL-mediated DC apoptosis through PI3K signaling, which is expected to facilitate the survival of DCs and promote the immune response against pathogens.

Keywords

Caspase, dendritic cell, fas ligand, PI3K and vitamin E.

References

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