Tran Thi Van Anh, Pham Quoc Tuan, Nguyen Van Khanh, Dao Viet Hung, Nguyen Thanh Hai, Nguyen Quoc Tuan, Nguyen Thi Minh Diep, Ngo Thi Xuan Thinh, Ha Thanh Hoa

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Kirenol (1), darutoside (2), and darutigenol (3), which are major active diterpenoids isolated from Herba Siegesbeckiae, are reported for analgesic activity and inflammatory effects. Currently, in Vietnamese Pharmacopoeia V, there is no requirement for the contents of active ingredients in this medicinal plant. In this study, a high-performance liquid chromatography (HPLC) method, which was developed with good repeatability and recovery, was used for simultaneous quantification of 1, 2, and 3 in Herba Siegesbeckiae. This method was successfully applied to quantify 1-3 in samples of Herba Siegesbeckiae collected from different locations. The results showed that the content of compound 1 accounted for 0 – 5.77 mg/g, compound 2 ranged from 0.82 to 5.48 mg/g, and compound 3 ranged from 0.37 to 3.18 mg/g. These results are considered a reference for upgrading the specification and quality control of Herba Siegesbeckiae.


Herba Siegesbeckiae; kirenol; darutoside; darutigenol; HPLC.