Analysis Characteristics of Genetic Alterations in Colorectal Cancer

Abstract

This study aims to evaluate the mutation status of KRAS, BRAF, NRAS, PIK3CA, and AKT and analyze the association of gene mutations with clinicopathological characteristics of colorectal cancer. 151 colorectal cancer patients were diagnosed at Pathology and Molecular Biology Center in National Cancer Hospital K. The research was conducted as a cross-sectional study using methods such as genomic DNA extraction; mutation analysis by Real-time PCR. Mutation rates of KRAS, BRAF, NRAS, PIK3CA, and AKT were 37.1%, 7.3%, 2.6%, 12.6%, and 1.3%, respectively. KRAS mutation was significantly associated with the size tumor. Additionally, KRAS mutation was mutually exclusive against that of BRAF mutation and tended to coexist with PIK3CA mutation (p<0.05). In conclusion, the most popular mutation in colorectal cancer was KRAS. These were different in gene mutations with clinicopathological characteristics as well as each mutation with others.