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Abstract: This study investigated the binding affinity of constitutive androstane receptor (CAR) with its activator, 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl) oxime (CITCO) in order to develop a rapid method for screening of protein-binding compounds. At first, the performance capacity of surface plasmon resonance (SPR) system was confirmed by the interaction of commercial carbonic anhydrate II (CAII) protein and 4-carboxybenzenesulfonamide (CBS) compound. The target protein, ligand binding domain of human CAR (hCAR), was optimally immobilized on SPR sensor chip using amine coupling method and then used to detect the interaction with chemical molecules. CITCO, known as a hCAR agonist in previous studies was used as the positive control to develop the method for determination of the binding affinities between SPR-immobilized CAR proteins and chemicals. As expected, CITCO showed specific bindings to hCAR protein in this study, indicating the application potential of SPR system in screening probable ligands of proteins.Keywords: Constitutive androstane receptor, CITCO, surface plasmon resonance.
 Wada, T., J. Gao, and W. Xie, PXR and CAR in energy metabolism, Trends Endocrinol. Metab. 20(6) (2009) p.273-279.
 Qatanani, M., J. Zhang, and D.D. Moore, Role of the Constitutive Androstane Receptor in Xenobiotic-Induced Thyroid Hormone Metabolism, Endocrinology 146(3) (2005) p. 995-1002.
 Min, G., Estrogen modulates transactivations of SXR-mediated liver X receptor response element and CAR-mediated phenobarbital response element in HepG2 cells, Exp. Mol. Med. 42(11) (2010) p.731-738.
 Moore, L.B., et al., Orphan Nuclear Receptors Constitutive Androstane Receptor and Pregnane X Receptor Share Xenobiotic and Steroid Ligands, J. Biol. Chem. 275(20) (2000) p.15122-15127.
 Sakai, H., et al., Transactivation Potencies of Baikal Seal Constitutive Active/Androstane Receptor by Persistent Organic Pollutants and Brominated Flame Retardants, Environ. Sci. Technol. 43(16) (2009) p.6391-6397.
 Yamamoto, Y., et al., The Orphan Nuclear Receptor Constitutive Active/Androstane Receptor Is Essential for Liver Tumor Promotion by Phenobarbital in Mice, Cancer Res. 64(20) (2004) p.7197-7200.
 Dong, B., et al., Activation of nuclear receptor CAR ameliorates diabetes and fatty liver disease, Proc. Natl. Acad. Sci. U.S.A. 106(44) (2009) p.18831-18836.
 Rich, R.L., et al., Kinetic analysis of estrogen receptor/ligand interactions, Proc. Natl. Acad. Sci. U.S.A. 99(13) (2002) p.8562-8567.
 Bravman, T., et al., Exploring "one-shot" kinetics and small molecule analysis using the ProteOn XPR36 array biosensor, Anal. Biochem. 358(2) (2006) p.281-288.
 Boaz Turner, M.T., and Shai Nimri, Applications of the ProteON GLH sensor chip: Interactions between Proteins and Small Molecules, Bio Rad tech note, 2008.
 Vered Bronner, T.B., Ariel Notcovich, Dana Reichmann, Gideon Schreiber, and Kobi Lavie, Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn™ XPR36 Protein Interaction Array System, Bio Rad tech note, 2006.
 Maglich, J.M., et al., Identification of a novel human constitutive androstane receptor (CAR) agonist and its use in the identification of CAR target genes, J. Biol. Chem. 278 (2003) p.17277 - 17283.