Method Development for Detection and Classification of Conjunctivitis-Causing Adenoviruses in Human

Nguyen Viet Ha, Nguyen Thi Thu Huyen, Do Thi Thanh Huyen, Nguyen Quang Hung, Tran Thuy Anh, Hoang Anh Tuan, Nguyen Van Sang

Article Sidebar

PDF
Published Mar 15, 2016

Article Details

How to Cite
HA, Nguyen Viet et al. Method Development for Detection and Classification of Conjunctivitis-Causing Adenoviruses in Human. VNU Journal of Science: Natural Sciences and Technology, [S.l.], v. 32, n. 1S, mar. 2016. ISSN 2588-1140. Available at: <https://js.vnu.edu.vn/NST/article/view/2825>. Date accessed: 23 nov. 2024.
ABNT APA BibTeX CBE EndNote - EndNote format (Macintosh & Windows) MLA ProCite - RIS format (Macintosh & Windows) RefWorks Reference Manager - RIS format (Windows only) Turabian
Issue
Vol 32 No 1S
Section
Review Articles

Main Article Content

Abstract

Abstract: Conjunctivitis or “pink eye” disease caused by human adenoviruses (HAdVs) is highly contagious and persistent morbidity without any effective treatments. Types of human adenoviruses (HAdV) are also very diverse. Therefore, precise determination of HAdV-types causing conjunctivitis is important for epidemiological studies. In this study, we aimed to develop a method for detection and classification of human adenoviruses causing conjunctivitis inVietnam. The HAdV genome was extracted from clinical samples of conjunctivitis patients inVietnam. The hypervariable region 7 (HVR-7) in hexon gene of adenoviruses was amplified with the redesigned primers. HVR-7 region was then sequenced to investigate and determine the HAdV-types. The HAdV types were identified by analyzing HVR-7 sequence of the hexon gene. In this research, HAdV-3, -4, -7, -8, -37 were identified as the cause of conjunctivitis, in which serotype 8 was the predominant type, detected in 19/23 samples.

Keywords: Human adenovirus, hypervariable region-7, type, conjunctivitis, PCR.

References

[1] Pihos, A.M., Epidemic keratoconjunctivitis: A review of current concepts in management. Journal of optometry, 2013. 6(2): p. 69-74.
[2] Ishii, K., et al., Comparative studies on aetiology and epidemiology of viral conjunctivitis in three countries of East Asia—Japan, Taiwan and South Korea. International journal of epidemiology, 1987. 16(1): p. 98-103.
[3] Jawetz, E., The story of shipyard eye. British medical journal, 1959. 1(5126): p. 873.
[4] Jin, X.-H., et al., Molecular epidemiology of adenoviral conjunctivitis in Hanoi, Vietnam. American journal of ophthalmology, 2006. 142(6): p. 1064-1066.
[5] Ghebremedhin, B., Human adenovirus: Viral pathogen with increasing importance. European Journal of Microbiology and Immunology, 2014. 4(1): p. 26-33.
[6] Acheson., N.H., Fundamentals of molecular virology. 2nd ed. 2011. 274-275.
[7] Norrby, E., The structural and functional diversity of adenovirus capsid components. Journal of General Virology, 1969. 5(2): p. 221-236.
[8] Pichla-Gollon, S.L., et al., Structure-based identification of a major neutralizing site in an adenovirus hexon. Journal of virology, 2007. 81(4): p. 1680-1689.
[9] Madisch, I., et al., Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy. Journal of virology, 2005. 79(24): p. 15265-15276.
[10] Avellón, A., et al., Rapid and sensitive diagnosis of human adenovirus infections by a generic polymerase chain reaction. Journal of virological methods, 2001. 92(2): p. 113-120.
[11] Sarantis, H., et al., Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing. Journal of Clinical Microbiology, 2004. 42(9): p. 3963-3969.
[12] Takeuchi, S., et al., Serotyping of adenoviruses on conjunctival scrapings by PCR and sequence analysis. Journal of Clinical Microbiology, 1999. 37(6): p. 1839-1845.