Direct Adventitious Shoot Regeneration from Triploid Watermelon Cotyledons
Main Article Content
Abstract
Abstract: An in vitro regeneration system was established for cotyledon explants of triploid watermelon (Citrullus lanatus Thumb.). Full strength MS medium supplemented with 0.2 mg.L-1 IAA in combination with different concentrations of BAP was employed for investigating adventitious shoot formation. Medium containing 1.5 mg.L-1 BAP and 0.2 mg.L-1 IAA was suitable for direct shoot induction from cotyledon explants. Adventitious shoots were elongated for 3 weeks on medium containing 0.5 mg.L-1 GA3 before transferring into rooting medium. Frequency of root induction achieved approximately 88% with an average of 4.3 root per shoot on half strength MS medium supplemented with 0.5 mg.L-1 IBA. Rooted shoots were successfully acclimatized in potted soil mixture with survival rate of 82.6%.
Keywords: Triploid, Citrullus lanatus, regeneration.References
[2] http://faostat.fao.org
[3] Li J., Li M., qin Y.G., Tang Y., Wang L., Ma C., and Li H.X., Optimized system for plant regeneration of watermelon (Citrullus lanatus Thumb). Afri J Biotech 10 (45) (2011) 9760-9765.
[4] Cho M.A., Moon C.Y., Liu J.R., and Choi P.S., Agrobacterium-mediated transformation in Citrullus lanatus. Bio Planta (55) (2008) 365-369.
[5] Guo S.G., Zhang J.G., Sun H.H., Salse J., Lucas W.J., Zhang H.Y., Zheng Y., Mao L.Y., Ren Y., and Wang Z., The draft genome of watermelon (Citrus lanatus) and resequencing of 20 diverse accessions. Nat Genet (45) (2013) 51-58.
[6] Marr c.W., and Gast K.L.B., Reactions by consumers in a farmer’s market to prices for seedless watermelon and ratings of eating quality. Hort Tech 1 (1991) 105-106.
[7] Shalaby T.A., Omran S.A., and Baioumi Y.A., In vitro propagation of two triploid hybrids of watermelon through adventitious shoot organogenesis and shoot tips culture. Acta Biol Szegediensis 52(1) (2008) 27-31.
[8] Murashige T. and Skoog F., A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3) (1962) 473-497.
[9] Zhao X., Niu X., and Fan M., Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.). Afri J Biotech 14(27) (2015) 2202-2210.
[10] Islam R., Hoque A., Rahman M.H., and Ahad A., Plant regeneration from hypochotyl explants of Citrullus lanatus Thumb. Pak J Bot 31(1) (1999) 31-35.
Badr-elden A.M., Nower A.A., Ibrahim I.A., Ebrahim M.K.H., and Abd Elaziem T.M., Minimizing the hyperhydricity associated with in vitro growth and development of watermelon by modifying the culture conditions. Afri J Biotech 11(35) (2012) 8705-8717.