Isolation, Selection and Biodegration Capability Investigation of Bacteria Chemotactic to Toluene
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Abstract
Abtract: Aromatic compounds resulted from untreated industrial waste is one of the causes of environmental pollution. The current treatment technology for these compounds is often expensive, complicated or not thorough, causing secondary pollution. Using bacteria chemotactic to pollutants in the detection and treatment of pollutants is a potential treatment measure that offer low cost and simple procedures. In this study, we aim to screen for bacterial strains capable of being chemotatic to toluene, as a model aromatic compound, and degrading it. The experiments testing the chemotaxis of bacteria to toluene were carried out on 0.2% agar minimal medium. The capability of tested bacteria in degrading toluene was experimented on minimal medium solid and semi-solid agar plates. We successfully isolated a strain, designated as HTD 3.12, from soil samples atTamDaoNational Park, that is attractively chemotactic to toluene and also capable of degrading it. The toluene-degrading ability of HTD 3.12 appeared to be more efficient due to its chemotatic activity to toluene.
Keywords: Bacterial chemotaxis, toluene, biodegradation.References
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