Assessment of Genetic Relationship of some Horseshoe Bats (Chiroptera: Rhinolophidae) in Vietnam Using Cytochromoxydase Subunit I (COI) Gene Sequence
Main Article Content
Abstract
Abstract: DNA barcoding was used to examining the genetic relationship between some Rhinolophus bat taxa (R. malayanus, R. cf. malayanus, R. marshalli, R. cf. marshalli) in Vietnam using cytochrome oxidase-I (COI) gene sequence. Through this study, we constructed the phylogenic trees and analysed genetic relationships between some Rhinolophus taxa collected in Vietnam. The obtained phylogenetic tree showed two well-defined clusters. The genetic distances between species varied from 2.7% to 16.3%. The smallest distances were recorded between species from the same group whereas the largest distances were between species from the different groups. Genetic data supported the previous conclusion based on morphological classification of R. malayanus, R. cf. malayanus, R. marshalli, R. cf. marshalli.
Keywords: Genetic relationship, COI gene, Rhinolophus, Vietnam.References
References
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H. T. Thanh, N. T. Son N. V. Khoi, V. D. Thong, Bat species composition in Ba Hon area, Hon Dat district, Kien Giang province. Proceeding of the 6th National Scientific Conference on Ecology and Biological Resources, Hanoi: (2015) 865 (in Vietnamese).
[1] S. Desmyter and M. Gosselin, COI sequence variability between Chrysomyinae of forensic interest, Forensic SciInt Genet, 3 (2009)89.
[2] S. Hemmerter, J. Slapeta and N.W. Beebe, Resolving genetic diversity in Australasian Culexmosquitoes: incongruence between the mitochondrial cytochrome c oxidase I and nuclear acetylcholine esterase 2, MolPhylogenetEvol, 50 (2009) 317.
[3] S. N. Kutty, T. Pape, A. Pont, B. M. Weigmann. and R. Meier, The musCOIdea (Diptera: Calyptratae) are paraphyletic: Evidence from four mitochondrial and four nuclear genes, MolPhylogenetEvol, 49 (2008) 639.
[4] R. D. Bradly, R. J. Baker, A test of the genetic species concept: cytochrome-b sequences and mammals, Journal of Mammalogy, 82(4) (2001) 960.
[5] P. D. N. Hebert, M. Y. Stoeckle, T. S. Zemlak, C. M. Francis, Identification of birds through DNA barcodes, PLoS Biology 2 (2004) e312.
[6] M. A. Smith, N. A. Poyarkov, P. D. Hebert, COI DNA barcoding amphibians: take the chance, meet the challenge, Molecular Ecology Resources 8 (2008) 235.
[7] M. Pfunder, O. Holzgang, J. E. Frey, Development of microarray-based diagnostics of voles and shrews for use in biodiversity monitoring studies, and evaluation of mitochondrial cytochrome oxidase I vs. cytochrome B as genetic markers, Molecular Ecology 13 (2004) 1277.
[8] C. M. Francis, A. V. Borisenko, N. V. Ivanova, J. L. Eger, B. K. Lim, A. Guillen-Servent, S. V. Kruskop, I. Mackie, P. D. N. Hebert, The Role of DNA Barcodes in Understanding and Conservation of Mammal Diversity in Southeast Asia, PLoS ONE 5(9) (2010) 1.
[9] V. D. Thong, S. J. Puechmaille, A. Denzinger, C. Dietz, G. Csorba, P. J. J. Betes, E. C. Teeling and H. U. Schnitzler, A new species of Hipposideros (Chiroptera: Hipposideridae) from Vietnam. Journal of Mammalogy, 93(1) (2012) 1.
[10] F. Mayer, O. Helversen, Criptic diversity in European bats, Proc. R. Soc. Lond. B 268 (2001) 1825.
[11] K. Sun, J. Feng, Z. Zhang, L. Xu, Y Liu, Criptic diversity in Chinese rhinolophids and hipposiderids (Chiroptera: Rhinolophidae and Hipposideridae), Mammalia, 73 (2009) 135.
[12] G. Li, G. Jones, S. J. Rossiter, S-F. Chen, S. Parsons, S. Zhang, Phylogenetics of small horseshoe bats from East Asia based on mitochondrial DNA sequence variation, Journal of Mammalogy, 87 (6) (2006)1234.
[13] E. L. Clare E.L., B. K. Lim, M. D. Engstrom, J. L. Eger J.L., Hebert P.D.N., DNA barcoding of Neotropical bats: species identification and discovery within Guyana. Molecular Ecology Notes 7 (2007) 184.
[14] P. Campbell, C. J. Schneider, A. M. Adnan, A. Zubaid, T. H. Kunz, Phylogeny and phylogeography of Old World fruit bats in the Cynopterusbrachyotis complex. Molecular Phylogenetics and Evolution 33 (2004) 764.
[15] V. D. Thong,, S. J. Puechmaille, A. Denzinger, P. J. J. Bates, C. Dietz, G. Csorba, P. Soisook, E. C. Teeling, S. Matsumura, N. M. Furey, H. U. Schnitzler, Systematics of the Hipposideros turpis complex and a description of a new subspecies from Vietnam. Mammal Rev., Volume 42, No. 2 (2012) 166.
[16] V. T. Tu, R. Cornette, J. Utge and A. Hassanin, First records of Murina lorelieae (Chiroptera: Vespertilionidae) from Vietnam. Mammalia 79(2) (2015) 201.
[17] V. T.Tu, G. Csorba, T. Gorfol, S Arai, N. T. Son, H. T. Thanh, and AlexandreHasanin, Description of a new species of the genus Aselliscus (Chiroptera, Hipposideridae) from Vietnam. Actachiropterologica, 17(2) (2015) 233.
[18] P. D. N. Hebert, A. Cywinska, S. L. Ball, J. R. deWaard, Biological identifications through DNA barcodes, Proc. R. Soc. Lond. B 270 (2003), 313.
[19] J. Sambrook J., D. R. Russell, Molecular Cloning: A laboratory manual,. 3rd ed, Cold Spring Harbor Laboratory Press, New York, 2001.
[20] N. V. Ivanova, J. R. deWaard, P. D/ N. Hebert, An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes 6 (2006) 998.
[21] http://blast.ncbi.nlm.nih.gov/Blast.cgi.
[22] K. Tamura, G. Stecher, D. Peterson, A. Filipski, and S. Kumar, MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30 (2013) 2725..
[23] A. Guillén-Servent, C. M. Francis, and R. E. Ricklefs, Phylogeny and biogeography of the horseshoe bats (Chiroptera: Rhinolophidae). Pp. xii-xiv in Horseshoe bats of the world (G.Csorba, P. Ujhelyi, and N. Thomas, eds.). Alana Books, Bishop’s Castle, United Kingdom, 2003.
H. T. Thanh, N. T. Son N. V. Khoi, V. D. Thong, Bat species composition in Ba Hon area, Hon Dat district, Kien Giang province. Proceeding of the 6th National Scientific Conference on Ecology and Biological Resources, Hanoi: (2015) 865 (in Vietnamese).