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A bioactive glass 46S6 with composition 46% SiO2 - 24% CaO - 24% Na2O - 6% P2O5 (wt%) was elaborated by melting method. ‘‘In vitro’’ bioactivity of bio-glass was evaluated by soaking of glass-powders in a simulated body fluid (SBF) at different times. The obtained results highlighted the bioactivity of the bio-glass by the formation of a bioactive hydroxyapatite (HA) layer on its surface. Experiments ''in vitro'' in the presence of cells confirmed the non-toxicity and the good cell viability on this bio-glass.