Survey on the presence of integrons in Edwardsiella ictaluri, the causative agent of bacillary necrosis on intensively farmed striped catfish (Pangasianodon hypophthalmus) in the Mekong Delta
Main Article Content
Abstract
The purpose of this research was to evaluate the presence of the integrons and characterise the gene cassette regions in E. ictaluri that causes bacillus necrosic of Pangasius on striped catfish (Pangasianodon hypophthalmus) in the Mekong Delta. By using PCR technique and gene sequencing, the finding identified 24/67 (35,82%) class 1 integron-positive E. ictaluri strains. Meanwhile, no class 2 and 3 integrons were detected in the study. Besides, our result also found many different gene cassette regions (0,65 kbp, 0,8 kbp, 0,95 kbp, 1,0 kbp, 1,2 kbp, 1,5 kbp and 2,5 kbp ) encoding to dihydrofolate reductase, aminoglycoside adenyltransferase, aminoglycoside N(6')-acetyltransferase and β-lactamase enzymes resistant to different antibiotics in E. ictaluri. The presence of the mobile genetic elements (class 1 integrons) in E. ictaluri in this research showed the ability that bacteria can transfer antimicrobial resistance genes to other bacteria in the aquatic environment. Therefore, using antibiotics for disease prevention in aquaculture should be managed more strictly
Keywords:
Antibiotic, Edwardsiella ictaluri, integron, Pangasianodon hypophthalmus
References
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Levesque, C., L. Piche, C. Larose and P.H. Roy, 1995. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrobial Agents and Chemotherapy, 39, 185-191.
Lin M., X. Wu, Q. Yan, Y. Ma, L. Huang, Y. Qin and X. Xu, 2016. Incidence of antimicrobial-resistance genes and integrons in antibiotic-resistant bacteria isolated from eels and aquaculture ponds. Diseases of Aquatic Organisms, 120: 115-123.
Lukkana, M., J. Wongtavatchai, and R. Chuanchuen, 2011. Class 1 Integrons in Aeromonas hydrophila isolates from farmed Nile Tilapia (Oreochromis nilotica). The Journal of Veterinary Medical Science, 4: 435-440.
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Mazel, D., B. Dychinco, V.A. Webb and J. Davies, 2000. Antibiotic resistance in the ECOR collection: integrons and identification of a novel aad gene. Antimicrobial Agents and Chemotherapy, 44: 1568-1574.
Miko A., K. Pries, A. Schroeter and R. Helmuth, 2005. Molecular mechanisms of resistance in multidrug-resistant serovars of Salmonella enterica isolated from foods in Germany. Journal of Antimicrobial Chemotherapy, 56: 1025-1033.
Mokracka J., R. Koczura and A. Kaznowski, 2012. Multiresistant Enterobacteriaceae with class 1 and class 2 integrons in a municipal wastewater treatment plant. Water Research, 46: 3353-3363.
Navia M.M., J. Gascon and J. Vila, 2005. Analysis of the mechanisms of resistance to several antimicrobial agents in Shigella spp. causing travellers’ diarrhoea. Clinical Microbiology and Infectious Diseases, 11: 1035-1047.
Nawaz, M., A.A. Khan, S. Khan, K. Sung, K. Kerdahi and R. Steele, 2009. Molecular characterization of tetracycline resistant genes and integrons from avirulent strains of Escherichia coli isolated from catfish. Foodborne Pathogens and Disease, 5: 553-559.
Ndi, L.O. and M.D. Barton, 2012. Resistance Determinants of Pseudomonas species from Aquaculture in Australia. Journal of Aquaculture Research & Development, 3:1-6.
Ndi, O.L. and M.D. Barton, 2011. Incidence of class 1 integron and other antibiotic resistance determinants in Aeromonas spp. from rainbow trout farms in Australia. Journal of Fish Diseases, 34: 589-599.
Nguyen H.N.K., V.T.T. Hao, N.H. Thinh, P.M. Smooker, J. Shimeta and P.J. Coloe, 2014. Molecular characterization of antibiotic resistance in Pseudomonas and Aeromonas isolates from catfish of the Mekong Delta, Vietnam. Veterinary Microbiology, 171: 397-405.
Nguyễn Thiện Nam, Phạm Thanh Hương, Trần Duy Phương và Từ Thanh Dung, 2010. Nghiên cứu sự đa kháng thuốc của vi khuẩn Edwardsiella ictaluri gây bệnh trên cá tra (Pangasianodon hypophthalmus). Tạp chí Khoa học Trường Đại học Cần Thơ, số 14b: 200-210.
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