Doan Thi Thuy Linh, Tran Thi Truong, Chu Hoang Ha, Le Van Son

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Abstract

Powdery mildew disease, one of the major diseases of soybean in Vietnam was caused by Microsphaera diffusa. SSR (Simple sequence repeats) marker, because of their co-dominance, polymorphic and stability have been wildely used to measure genetic diversity in crop. In the present study, genetic basis of 36 soybean genotypes varying in powdery mildew resistance was studied using 14 SSR markers. A total of 61 alleles with an average of 4,36 allele/locus, which indicated hight genetic diversity of the studied genotypes. PIC value of each marker ranged from 0 (Sat_396, Satt183, Sat_298) to 0,711 (Sat_224) with an average of 0.364 which shown high genetic diverrsity among the studied genotypes. At genetic similarity coefficient of 0.75, 36 soybean genotypes divided into 8 distint groups. Group 1 and group 2 included 21/25 genotypes which was resistant to powdery mildew. Groups 1, 2, 4, 5 and 8 include powdery mildew resistance genotypes.


 

Keywords: genetic diversity, Glycine max L., powdery mildew, resistance gene, SSR markers

References

[1] Rani, A., Kumar, V., Gill, B. S., Morya, V., & Rawal, R., Assessment of genetic diversity of soybean genotypes differing in resistance against yellow mosaic virus using simple sequence repeat markers, Legume Research 39 5 (2016) 674
[2] Tantasawat P., Trongchuen J., Prajongjai T., Jenweerawat S. & Chaowiset W., SSR analysis of soybean (Glycine max (L.) Merr.) genetic relationship and variety identification in Thailand, Australian Joural of Crop Science Vol. 5 3 (2011) 283
[3] Song, Q., Jia, G., Zhu, Y., Grant, D., Nelson, R. T., Hwang, E. Y., ... & Cregan, P. B., Abundance of SSR Motifs and development of Candidate Polymorphic SSR Markers (BARCSOYSSR_1.0) in Soybean. Crop Sci. 50 (2010) 1950
[4] Triệu Thị Thịnh , Vũ Thị Thúy Hằng, Vũ Đình Hòa, Phân tích đa dạng di truyền của đậu tương bằng chỉ thị SSR, Tạp chí Khoa học và Phát triển, tập 8 4 (2010) 638
[5] Vũ Thanh Trà, Chu Hoàng Mậu, Trần Thị Phương Liên, Đánh giá sự đa dạng di truyền của 50 giống đậu tương Việt Nam có phản ứng khác nhau với bệnh gỉ sắt bằng chỉ thị SSR, Tạp chí Sinh học, tập 34 2 (2012) 235
[6] Bisen, A., Khare, D., Nair, P., & Tripathi, N., SSR analysis of 38 genotyles of soybean (Glycine max (L.) Merr.) genetic diversity in India, Physiol Mo Biol Plants (2014)
[7] Saghai-Maroof, K., Soliman, M., Jorgensen, R.A., & Allard, R.W., Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics, Proceedings of the National Acafemy of Sciences USA, Vol. 81 24 (1984) 8014
[8] Nei M., Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA, 70 (1973) 3321
[9] Tuong, H. M., Truong, T. T., Lien, T. T. P., & Ha, C. H., Analysis of Powdery Mildew Resistant Characteristic and Genetic Relationship of Cultivated Soybean, Glycine max, in Vietnam for Parental Selection. International Journal of Bioscience, Biochemistry and Bioinformatics, 6 (3) (2016) 105
[10] Trần Thị Phương Liên, Lê Thị Muội, Nghiên cứu về sự đa dạng di truyền của một số giống đậu tương bằng chỉ thị phân tử SSR, Tạp chí Công nghệ Sinh học, tập 1 3 (2003) 347