Agricultural production is now severely affected by pathogenic microorganisms including molds. The use of chemical fungicides for controlling plant pathogens has negatively influenced on the quality of agricultural products and the environment. Currently, biological control using antifungal preparations is one of the top priorities in the production of safe agricultural products. In this study, 254 bacterial strains were isolated from the plant rhizosphere, of which 11 isolates exhibited antagonistic properties against several fungal pathogens including Fusarium oxysporum, Phytophthora capsici, Sclerotium hydrophila, Neoscytalidium dimidiatum, Aspergillus flavus and Aspergillus niger. These bacterial strains were identified as Bacillus subtilis, Bacillus amyloliquefaciens and Burkholderia vietnamienis by sequencing of 16S rRNA genes. The Bacillus isolates are capable of producing extracellular enzymes to decompose starch, casein and chitin. The strains obtained in this study could be used to supplement microbial preparations or organic fertilizers to replace pesticides, contribute to environmental protection and improve quality of agricultural products.