Clonal propagation of Zingiber officinale (Willd.) Roscoe through in vitro explant cultures
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Abstract
Ginger is a plant product in Vietnam as well as in many Asian countries with the main purpose of making spices and medicines. This study focuses on determining the conditions in some key steps to accelerate the in vitro breeding of Gingerbreads by transplant cell culture. Initial results are presented as below: In the sterilize stage, sample was sterilized by NaClO (20%) in 15 minutes which is considered as the most suitable antiseptic formula with the rate of alive sample is 63%. Move to the explant culture stage in MS environment, 3mg/I BAP is added which is at the best rate of shoot growth and pre-shoot growth. It then will be the important material for fast breeding process. In addition, vitamin B1 is added as well for better plant growth, 1,0mg/lBAP, 0,1mg/l Ki and 3mg/lB1 for particular. The last stage is complete developmental environment with 3 mg/L B1 and 0,3 mg/l NAA are added, it would be the most efficient environment for the development of the root system after 1 month.
References
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