Cloning and co-expression of genes encoding two types of GP5ecto envelope antigens (ectodomain) and M of pathogenic porcine reproductive and respiratory syndrome virus
Main Article Content
Abstract
Porcine Reproductive and Respiratory Syndrome (PRRS) is a common infectious disease in the pig industry, caused by the PRRSV virus. GP5 protein (ORF5) encodes the outer skin glycoprotein, including the extracellular (ectodomain) and endothelium (endodomain). GP5 and M proteins are tightly bound together by disulfide bridges to assemble and infect the virus. Co-expression of GP5 and M proteins can develop a stronger immune response. In this study, the two genes coding for the GP5ecto protein (ectodomain) and M of the PRRSV strain (VN07196) were amplified individually, which were then coupled together using PCR technique. Using specific 35S, Histag and Cmyc promoters, ELPs, clones in the pRTRA vector and designed into the pCB301 plant transformation vectors. The ELP-binding GP5ecto-M binding protein was successfully demonstrated in the tobacco leaves of N. benthamiana by the clever, optimal and purified gene expression technology of GP5ecto-M by the mITC method. Succeeded this protein. This is an important scientific basis for the large-scale demonstration and purification of GP5ecto-M antigens for the purpose of studying the immunogenicity in animals and the production of PRRSV subunit vaccine.
References
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