Biological characteristics of strain Paecilomyces variotii NV01 isolated in Đăk Lăk pepper soil
Main Article Content
Abstract
From 15 pepper soil samples in Daklak, based on chitinase, protease, amylase, and cellulase activities, strain NV01 was chosen for further research. Selected strains NV01 were studied for morphology, colonial color, nutritive influences to spore formation; It has been shown that the PDA environment is suitable for the growth of strain NV01. After 14 days of inoculation, this fungus sporulated 106 conidia x cm-1. The strain is capable of producing some extracellular enzymes such as amylase, cellulase, chitinase, and protease. The sequence-based results ITS1F and ITS4 showed that the strains were highly similar (100%) to Paecilomyces variotii KF305752 and were assigned as Paecilomyces variotii NV01.
In Vietnam, there is very little research into the this fungus, and databases of biological characteristics are still limited.
Keywords:
Biological characteristics, Paecilomyces variotii, pepper
References
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[5] Bauer A W, Kirby W M M, Sherris J C & Turck M. Antibiotic susceptibility testing by a standardized single disk method. Amer. I. C/in. Pathol. Depts. Microbiology and Medicine, Univ. Washington, Sch. Med., Seattle. 493-6, (1966) 45.
[6] Luangsa-ard J. J., N. L. Hywel-Jones, L.Manoch, R. A. Samson, On the relationships of Paecilomyces sect, Isarioidea species. Publisher. British Mycological Society. Vol 109 (5), (2005), pp 581 – 589.
[7] Juan Li, Chenggang Zou, Jianping Xu, Xinglai Ji, Xuemei Niu, Jinkui Yang, Xiaowei Huang, and Ke-Qin Zhang, Molecular Mechanisms of Nematode-Nematophagous Microbe Interactions: Basis for
Biological Control of Plant-Parasitic Nematodes. Phytopathol. (2015) , 53.
[8] Khan A, Williams KL, Nevalainen HKM, Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juveniles, Biological Control 31, (2004), 346–352.
[9] Van-Nam Nguyen • In-Jae Oh • Young-Ju Kim •Kil-Yong Kim • Young-Cheol Kim • Ro-Dong Par J Ind. Purification and characterization of chitinases from Paecilomyces variotii DG-3 parasitizing on Meloidogyne incognita eggs, (2009), 195–203
[10] Chen, Y. Y., Cheng, C.Y., Huang, T. L.,. Chitosanase from Paecilomyces lilacinus with binding affinity for specific chitooligosacharide. Biotechnology and Applied Biochemistry, (2005), 145-150.
[11]Segers, R., Butt, T. M., Keen, J.N., Kerry, B. R., & Peberdy, J. F. The Subtilisins of the invertebrate mycopathogens Verticillium chlamydosporium and Metarhizium anisopliae are serologically and functionally related. FEMS Microbiology Letters, 126, (1995), 227 – 232.
[12] Zaldivar M, Velasquez JC, Contreras I, Perez LM Trichoderma aureoviride 7–121, a mutant with enhanced production of lytic enzymes: its potential use in waste cellulose degradation and/or biocontrol. Electron J Biotechnol, (2001), 1–9
[13] Zeilinger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M, Kubicek CP. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet Biol., (1999), 131–140.
[14] Kang SC, Park S, Lee DG. PuriWcation and characterization of a novel chitinase from the entomopathogenic fungus, Metarhizium anisopliae. J Invertebr Pathol. (1999), 276–281.
[15] Leger S, Joshi RJ, Bidochka L, Roberts DW. Characterization and ultrastructural localization of Metarhizium anisopliae, M. Xavoviride, and Beauveria bassiana during fungal invasion of host (Manduca sexta) cuticle. Appl Environ Microbiol, (1999), 907–912
[16] M. Al-Qasim, W. Abu-Gharbieh and K. Assas. Nematophagal ability of Jordanian isolates of Paecilomyces variotii on the root-knot nematode Meloidogyne javanica. Nematol. medit. (2009), 53-57
[17] Z. Perveen and S. Shahzad. A comparative study of the efficacy of Paecilomyces species against root-knot nematode Meloidogyne incognita Pakistan Journal of Nematology, 2013, Vol. 31 (2), 125-131