Do Thi Thanh Huyen, Tran Thi Thuy Anh, Nguyen Thi Hong Van, Nguyen Quang Huy, Nguyen Van Sang

Main Article Content

Abstract

NF-κB is a transcription factor, which plays a central role in regulating the immune system. It controls up to 500 genes involved in immune responses and also coordinates both innate and adaptive immunity. NF-κB participates in cellular responses to diverse stimuli such as stress, cytokines, free radicals, ultraviolet radiation, and bacterial or viral antigens. Incorrect regulation of NF-κB has been linked to a number of human diseases such as cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. Among human NF- κB transcription factors, NF- κB p50 is one of the most important transcription factor which is found in all type of cells. In this study, we cloned the gene encoding for human NF-κB1 p50 and inserted the gene in to pET-M expression vector (modified form of pET-32a expression vector). Vector pET-M containing NF-κB1 p50 gene was transformed and expressed in BL21(DE3) E.coli cells. Recombinant NF-κB1 p50 protein was purified using nikel bead column with the purity close to 99%. As a result the obtained NF-κB1 p50 with high purity can be used for testing and screening of potential drug for NF-κB related diseases.

Keywords: gene expression, recombinant protein, NF-κB, affinity chromatography

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