Identification of seasonal influenza virus in the Northern Vietnam, 2013-2015
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Abstract
Monitoring of influenza virus subtypes has provided the useful information for the Global Influenza Surveillance and Response System (GISRS) in influenza vaccines development to improve vaccine efficacy. A number of influenza A/H1N1pdm09, A/H3N2 and B viruses by RT-PCR from human clinical specimens collected from 2013 to 2015 in Northern Vietnam were isolated on MDCK-SIAT1 cells and identified by hemagglutination inhibition assay (HAI). The data reported here confirmed that MDCK-SIAT1 cells could be used efficiently in order to isolate influenza A/H1N1pdm09, A/H3N2 and B viruses with an average isolation rate of 59.25%. All A/H3N2 viruses were better recovered from MDCK-SIAT1 cells with low viral titer than influenza A/H1N1pdm09 and influenza B viruses. Almost all Vietnamese isolates were inhibited by ferret antisera raised against reference viruses with similar HAI titer recommened by WHO annually. A small proportion of viruses showed changed reactivity by HAI, reduced of virus neutralization in all influenza subtypes, in which the influenza A/H3N2 virus had the highest number: 2013 (4.9%), 2014 (1.9%) and 2015 (8.9%).
References
[2] Klimov AI, Garten R, Russell C et al. WHO recommendations for the viruses to be used in the 2012 Southern Hemisphere Influenza Vaccine: epidemiology, antigenic and genetic characteristics of influenza A(H1N1)pdm09, A(H3N2) and B influenza viruses collected from February to September 2011. Vaccine 2012; 30:6461–6471.
[3] Flannery B, Zimmerman RK, Gubareva LV, Garten RJ, Chung JR, et al. Enhanced genetic characterization of influenza A(H3N2) viruses and vaccine effectiveness by genetic group, 2014–2015. J Infect Dis. 2016;214(7):1010-1019.
[4] Veljkovic V, Paessler S, Glisic S, Prljic J, Perovic VR et al. Evolution of 2014/15 H3N2 Influenza viruses circulating in US: Consequences for vaccine effectiveness and possible new pandemic. Front Microbiol. 2015;6:1456.
[5] Oh DY, Barr IG, Mosse JA, Laurie KL.MDCK-SIAT1 cells show improved isolation rates for recent human influenza viruses compared to conventional MDCK cells. J Clin Microbiol. 2008 Jul;46(7):2189-94. doi: 10.1128/JCM.00398-08.
[6] Abdoli A, Soleimanjahi H, Jamali A, Mehrbod P, Gholami S, Kianmehr Z, Feizi N, Saleh M3, Bahrami F, Mokhtari-Azad T, Abdoli M, Kheiri MT.Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production. June 2016, Volume 38, Issue 6, pp 941–948
[7] Vuong D Cuong, Phuong M V Hoang, Hang L K Nguyen, Hien T Nguyen, Thach C Nguyen, Thanh T Le, David T Dennis, Bryan K Kapella, James C Kile, Mai Q Le (2012) “The genetic match between vaccine strains and circulating seasonal influenza A viruses in Vietnam, 2001-2009”, Influenza and Other Respiratory Viruses, DOI: 10,1111/irv.12038
[8] Hoàng Vũ Mai Phương, Lê Thị Thanh, Nguyễn Cơ Thạch, Ứng Thị Hồng Trang, Vương Đức Cường, Phạm Thị Hiền, Nguyễn Phương Anh, Trần Thu Hương, Hoàng Thu Hương, Nguyễn Vũ Sơn, Nguyễn Lê Khánh Hằng. Đặc điểm phân đoạn gen HA và NA virut cúm mùa gây bệnh trên các bệnh nhân viêm phổi nặng nghi do virut thu thập tại một số bệnh viện miền Bắc Việt Nam, 2013-2015. Tạp chí Y học dự phòng, tập XXVI, số 10 (183), tr 107-118.