Genetic diversity of some strains of Staphylococcus aureus isolated from kitchens at primary schools in Hanoi in 2015
Main Article Content
Abstract
Food poisoning is always a hot issue not only in Vietnam but also in the world. Investigation causes, origin of factors causing food poisoning are always concerned by the authorities. In this study, we used the PFGE method to analyze the genetic diversity and relationships of Staphylococcus aureus isolates, one of the leading causes of mass food poisioning. S. aureus strains were isolated in kitchens at some primary schools in Hanoi in 2015.
The study was conducted on 68 isolates from samples of the food handlers,equipments, foods, from 36 primary schools in 12 districts of Ha Noi. The results showed that the proportion of S. aureus carrying enterotoxigenic genes (sea,seb,sec) was 20.6% (n = 68). 46 isolates from 18 schools with high genetic diversity: 8 clusters with a similarity coefficient of ≥80% and cross-contamination between samples in the same school were happened (between handlers-foods- processing equipment).
Keywords:
Staphylococcus aureus, food safety, food safety at kitchens, Kitchen of primary school at Hanoi.
References
1. Nguyễn Thị Giang, Nguyễn Thành Trung, Đặng Thị Oanh, Tạ Thị Yến, Lê Thị Hồng Hảọ, Phạm Xuân Đà (2015),"thực trạng ô nhiễm Staphylococcus aureus trong bếp ăn tập thể tại một số trường tiểu học bán trú trên địa bàn Hà Nội năm 2015", Tạp chí y học thực hành, số 8/2016, trang128.
2. Lê Khánh Trâm (2013), "Xác định kiểu cách cư trú và gen độc lực của Staphylococcus aureus ở nhóm người làm việc tại một số cơ sở dịch vụ ăn uống". LATS y học
3. A. Stranden, R.Frei, A.F. Widmer (2003), "Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR replace Pulsed-Field Gel Electrophoresis", Journal of Clinical Microbiology p.3181-3186.
4. Akira Shimizu, Manabu Fujita et al (2000), “Characterization of Staphylococcus aureus Coagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreak (1980-1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis”, Journal of Clinical Microbiology, p.3746-3749.
5. Argudin MA, Mendoza MC, Rodicio MR (2010) Food poisoning and Staphylococcus aureus enterotoxins. Toxins 2(7):1751–1773
http://www.fda.gov/Food/FoodborneIllnessContaminants/CausesOfIllnessBadBugBook/ucm2006773.htm
6. Christiane schlichting, Catherine Branger et al (1993), "Typing of Staphylococcus aureus by Pulsed-Field Gel Electrophoresis, Zymotyping, Capsular Typing, and Phage Typing: Resolution of Clonal relationships", Journal of Clinical Microbiology, p.227-232.
7. Dana A.Bazzoun, Houda H.Harastani et al (2014), "Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan", J Infect Dev Ctries, 441-447. doi 10.3588\jidc.3676.
8. Fred C. Tenover, Robert D. Arbeit, Richard V.Goering et al (1995), Interpreting Chromosomal DNA Restriction Patterns Produced by Pulsed- Field Gel Electrophoresis: Criteria for Bacterial Strain Typing", Journal of Clinical Microbiology,p.2233-2239.
9. FDA (2012) Bad bug book: Foodborne pathogenic microorganisms and natural toxins handbook, 2nd ed. US Food and Drug Administration, Silver Spring, p. 87–92.
10. Ghosh M, Wahi S, Kumar M, Ganguli A (2007),”Prevalence of enterotoxigenic Staphylococcus aureus and Shigella spp. in some raw street vended Indian foods”, Int J Environ Health Res.Apr;17(2):151-6.
11. H-L Wei and C-S.Chiou (2001), "Molecular subtyping of Staphylococus aureus from an outbreak associated with a food handler", Epidemiol Infect, 128,15-20.
12. Hyeon, Ji-Yeon, Gyung-Tae Chung et al (2013), "A Foodborne Outbreak of Staphylococcus aureus Associated with Fried Chicken in Republic of Korea", Journal Microbiol Biotechnol, 85-87.
13. I. Montesinos, E.Salido, T. Delgado et al (2002), "Epidemiologic Genotyping of Methicillin- Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms", Journal of Clinical Microbiology, p.2119-2125.
14. Sonja Bonness, Christiane Szekat et al (2008), “Pulsed-Field Gel Electrophoresis of Staphylococcus aureus Isolates from Atopic Patients Revealing Presence of Similar Strains in Isolates from Children and Their Parents”, Journal of Clinical Microbiology, p.456-461.
15. Tormod Mord, Tore Tollersrud et al (2005), "Genitic diversity of Staphylococcus aureus isolated from ovine intramamary infections in Norway", Veterinary Microbiology, p.265-273.
16. Yves Le Loir, Florence Baron and Michel Gautier (2003), Staphylococcus aurreus and food poisoning. Genetic and Molecular Research 2, pp.63-76.
2. Lê Khánh Trâm (2013), "Xác định kiểu cách cư trú và gen độc lực của Staphylococcus aureus ở nhóm người làm việc tại một số cơ sở dịch vụ ăn uống". LATS y học
3. A. Stranden, R.Frei, A.F. Widmer (2003), "Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR replace Pulsed-Field Gel Electrophoresis", Journal of Clinical Microbiology p.3181-3186.
4. Akira Shimizu, Manabu Fujita et al (2000), “Characterization of Staphylococcus aureus Coagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreak (1980-1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis”, Journal of Clinical Microbiology, p.3746-3749.
5. Argudin MA, Mendoza MC, Rodicio MR (2010) Food poisoning and Staphylococcus aureus enterotoxins. Toxins 2(7):1751–1773
http://www.fda.gov/Food/FoodborneIllnessContaminants/CausesOfIllnessBadBugBook/ucm2006773.htm
6. Christiane schlichting, Catherine Branger et al (1993), "Typing of Staphylococcus aureus by Pulsed-Field Gel Electrophoresis, Zymotyping, Capsular Typing, and Phage Typing: Resolution of Clonal relationships", Journal of Clinical Microbiology, p.227-232.
7. Dana A.Bazzoun, Houda H.Harastani et al (2014), "Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan", J Infect Dev Ctries, 441-447. doi 10.3588\jidc.3676.
8. Fred C. Tenover, Robert D. Arbeit, Richard V.Goering et al (1995), Interpreting Chromosomal DNA Restriction Patterns Produced by Pulsed- Field Gel Electrophoresis: Criteria for Bacterial Strain Typing", Journal of Clinical Microbiology,p.2233-2239.
9. FDA (2012) Bad bug book: Foodborne pathogenic microorganisms and natural toxins handbook, 2nd ed. US Food and Drug Administration, Silver Spring, p. 87–92.
10. Ghosh M, Wahi S, Kumar M, Ganguli A (2007),”Prevalence of enterotoxigenic Staphylococcus aureus and Shigella spp. in some raw street vended Indian foods”, Int J Environ Health Res.Apr;17(2):151-6.
11. H-L Wei and C-S.Chiou (2001), "Molecular subtyping of Staphylococus aureus from an outbreak associated with a food handler", Epidemiol Infect, 128,15-20.
12. Hyeon, Ji-Yeon, Gyung-Tae Chung et al (2013), "A Foodborne Outbreak of Staphylococcus aureus Associated with Fried Chicken in Republic of Korea", Journal Microbiol Biotechnol, 85-87.
13. I. Montesinos, E.Salido, T. Delgado et al (2002), "Epidemiologic Genotyping of Methicillin- Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms", Journal of Clinical Microbiology, p.2119-2125.
14. Sonja Bonness, Christiane Szekat et al (2008), “Pulsed-Field Gel Electrophoresis of Staphylococcus aureus Isolates from Atopic Patients Revealing Presence of Similar Strains in Isolates from Children and Their Parents”, Journal of Clinical Microbiology, p.456-461.
15. Tormod Mord, Tore Tollersrud et al (2005), "Genitic diversity of Staphylococcus aureus isolated from ovine intramamary infections in Norway", Veterinary Microbiology, p.265-273.
16. Yves Le Loir, Florence Baron and Michel Gautier (2003), Staphylococcus aurreus and food poisoning. Genetic and Molecular Research 2, pp.63-76.