Optimizing culture medium composition with added olive oil for isolation of extracellular polysaccharides in Ophiocordyceps sinensis fungus
Main Article Content
Abstract
Ophiocordeps sinensis is a medicinal mushroom which is highly valued in traditional and modern medicine. In addition, exopolysaccharides is a bioactivities as well as antioxidant, anti-cancer, anti-hypertensive, immunomodulatory and cholesterol-lowering. Therefore, O. sinensis cultured to improve EPS synthesis is essential. In this research, O. sinensis cultured in liquid medium with olive oil from 1 to 10% (v/v) to choose the oil concentration for the growth and EPS synthesis of O. sinensis. The results showed that the EPS were 5,03 ± 0,38 g/L (an increase of 2,94 times than controls sample) when added 5% olive oil to the culture medium. The suitable time to receive EPS is 40 days. Then, we used the Plackett – Burman experimental design, and saccharose (48,69 g/L), peptone (6,77 g/L) and olive oil (5,27%) were found to be the most significant parametes. The optimum conditions including saccharose, peptone and olive oil were determined by Box – Behnken method and the results showed maximum of EPS value is 6,06 g/L.
References
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