Study effect of nano metallic fertilizers on Pseudomonas sp.52
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Abstract
Today, research on the use of micro-material for use in fertilizer production for agriculture and less toxic cytotoxicity in humans and other microorganisms has become a trend. In this paper we present some results of the influence of metallic nanoparticles (Cu, ZnO, MnO2, B2O3, Fe and MoO3) on the growth of strain Pseudomonas sp. 52. Metallic nano-foliar fertilizers did not affect protease, amylase and cellulase protease activity of Pseudomonas sp. 52 at a concentration of 10 μg/ml enzyme. Metallic nanoparticles do not inhibit the growth of Pseudomonas sp. 52 concentrations at 10-200 μg/ml used agar diffusion plate method. Strain Pseudomonas sp. 52 at culture conditions of 150 rpm and temperature of 30°C, with metallic nanoparticles fertilizer concentration of 10 and 100 μg/ml inhibited of growth during the first 3 hours; At pH 4, Strain Pseudomonas sp. 52 is more powerful inhibited is at pH = 7 and pH = 10. Transmission electron microscopy analysis showed that Pseudomonas sp. 52 cells were damaged after contact with metallic nanoparticles fertilizer, causing cell contents to leak out
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