Potential applications of silver and copper nanoparticles for the control of Penicillium digitatum causing green mould disease on orange fruits in Vietnam
Main Article Content
Abstract
Penicillium digitatum is a major source of post-harvest decay on orange fruits. In Vietnam, the control of P. digitatum has been done mostly with use of chemicals which advertly affect local environment and human health. Recently, nanomaterials show significant effectiveness in treating pathogenic fungi without harming the environment and human health. Therefore, nanomaterials are regarded as promising agents in plant protection and post-harvest processing. In this study, a fungus labeled as N11 causing green mould disease was isolated from orange fruits of Tuyen Quang province and identified as Penicillium digitatum N11. The fungus was subjected to treatment with silver and copper nanoparticles and nanocomposites to evaluate the effectiveness of those materials in inhibition of fungal growth. Various concentrations of the nanoparticles were tested at different growth stages of the fungus. For silver and copper nanoparticles, the inhibition of fungal growth up to 50% was observed for 21 days at the concentrations of 200 ÷ 400 ppm and 250 ppm, respectively. At 500 ppm, copper nanoparticles completely inhibited the growth of Penicillium digitatum N11. Nano composite Ag-silica (AgS) showed stronger inhibition of fungal growth than Ag-Bentonite (AgB) nanocomposite. In the treatment of fungal spores, inhibition of germination up to 90% for three days was observed at the concentrations of 1000 ppm for Cu, above 200 ppm for Ag nanoparticles, over 2000 ppm of AgS nanocomposite and 4000 ppm of AgB nanocomposite
References
[2] Akhtar N., Tanjum T., Jabeen R., Isolation and Identification of Storage Fungi from Citrus Sampled from Major Growing areas of Punjab, Pakistan, Int J Agric Biol, Vol. 15, (2013): 1283‒1288.
[3] Yin G., Zhang Y., Pennerman K. K., Wu G., Hua S. S. T., Yu J., Jurick W. M. II, Guo A., Bennett J. W., Characterization of Blue Mold Penicillium Species Isolated from Stored Fruits Using Multiple Highly Conserved Loci, J Fungi ,Vol. 3, No. 1, (2017): 12.
[4] Roduner E., Size matters: why nanomaterials are different, Chemical Society Review, Vol. 35, (2006): 583-592.
[5] Jafari A., Pourakabar L., Farhadi K., Lida M. L., Goosta Y., Biological synthesis of silver nanoparticles and evaluation of antibacterial and antifungal properties of silver and copper nanoparticles, Turkish Journal of Biology,Vol. 39, (2015): 556-56.
[6] Tarafdar J. C., Sharma S, Raliya R., Nanotechnology: Interdisciplinary science of applications, Afr J Biotechnol, Vol. 12, (2013):219-226.
[7] Stoimenov P. K., Metal oxide nanoparticles as bactericidal agents, Langmuir, Vol. 18, (2002):6679-86
[8] Gavanji S., The effects of silver Nano particles on microorganisms: A review, Applied Science Reports, Vol. 1, No. 2, (2013): 50-56.
[9] Pitt J. I., Laboratory guide to common Penicillium species, Commonwealth Scientific and Industrial Research Organization. Food Research laboratory (1991), N.S.W. Australia.
[10] Frisvad J. C. and Samson R. A., Polyphasic taxonomy of Penicillium subgenus Penicillium: A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins, Studies in Mycology, Vol. 49, (2004): 1-174.
[11] El-Gali Z. I., Control of Penicillium digitatum on Orange Fruits with Calcium Chloride Dipping, Journal of Microbiology Research and Reviews, Vol. 2, No. 6, (2014): 54-61.
[12] Ni H-F., Yang H-R, Chen R-S., Liou R-F., Hung T-H., New Botryosphaeriaceae fruit rot of mango in Taiwan: identification and pathogenicity, Botanical Studies (2012), Vol. 53, (2012): 467-478.
[13] Andrew J.M., Determination of minimum inhibitory concentrations, Journal of Antimicrobial Chemotherapy, Suppl S1, Vol. 48, (2001): 5-16.
[14] Abdel-Hafez S. I. I., Nafady N. A., Abdel-Rahim I. R., Shaltout A. M., Daro J.-A., Mohamed M. A., Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani, 3 Biotech, Vol. 6, (2016): 199, DOI 10.1007/s13205-016-0515-6.
[15] Kim K.. J.., Sung W. S., Moon S. K.; Choi J. S., Kim J. G., Lee D. G., Antifungal effect of silver nanoparticles on dermatophytes, Journal of microbiology and biotechnology, Vol. 18, No. 8, (2008): 1482- 1484.
[16] Kim S. W., Jung J. H., Lamsal K, Kim K. S., Min J. S, Lee Y. S., Antifungal Effects of Silver Nanoparticles (AgNPs) against Various Plant Pathogenic Fungi, Mycobiology, Vol. 40, No. 1, (2012): 53–58.
[17] Mahdizadeh V., Safaie N., Khelghatibana F., Evaluation of antifungal activity of silver nanoparticles against some phytopathogenic fungi and Trichoderma harzianum, J Crop Prot, Vol. 4, No 3, (2015): 291-300.
[18] Kanhed, P. et al., In vitro antifungal efficacy of copper nanoparticles against selected crop pathogenic fungi, Mat. Lett. 115, (2014): 13-17.
[19] Pham Van Viet, Hai Thi Nguyen, Thi Minh Cao, and Le Van Hieu, Fusarium Antifungal Activities of Copper Nanoparticles Synthesized by a Chemical Reduction Method, Journal of Nanomaterials, Volume 2016, (2016).
[20] Chen P. S., Peng Y. H., Chung W. C., Chung K. R., Huang H. C. and Huang J. W., Inhibition of Penicillium digitatum and Citrus Green Mold by Volatile Compounds Produced by Enterobacter cloacae, J Plant Pathol Microbiol, (2016) 7:339.
[21] Cunningham N. M.& Taverner P. D., Efficacy of integrated postharvest treatments against mixed innoculations of Penicillium digitatum and geotrichum citriaurantii in ‘leng’ navel oranges (citrus sinensis), New Zealand Journal of Crop and Horticultural Science, Vol. 35, No. 2, (2007), 187-192