Nguyen Bao Ngoc, Do Thi Ly, Esther Derouet, Nguyen Huu Tuan Dung, Nguyen Thanh Tung, Nguyen Thi Phuong Linh, Nguyen Hoang Nam, Nguyen Minh Hieu, Nguyen Dinh Thang, Nguyen Thi Van Anh, Pham Thi Thu Huong

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Abstract: This research aims to develop and characterize a protein drug delivery system in agarose-glucan complex. The complex was produced by sonicating the mixture of agarose-glucan components and a protein in liquid paraffin with Sonics Vibracell Processor adapted from the method of Nuo Wang et all 1997. Etanercept, an anti-tumor necrosis factor-alpha (anti-TNF-α), was used as a model protein drug which was successfully encapsulated into agarose-glucan complex system. This protein can neutralize the TNF-α, a pro-inflammatory cytokine that plays a pivotal role in regulating the inflammatory response in rheumatoid arthritis (RA) and is well-known as mediator worsening RA pathogenesis. The prepared agarose-glucan complex possesses a range of sizes, from 30 to 150 nm, dissolves well within the range of pH buffer from 5.2 to 6.2 with an average protein encapsulating efficiency up to 74.4%, and can release up to 50% of protein after 40.3 hours. This research is the basis for developing nanogel-sized targeted drug delivery in RA treatment.

Keywords: Agarose gel, agarose microspheres, glucan, emulsification cooling, rheumatoid arthritis.

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