Nguyen Duc Dien, Nguyen Le Ai Vinh, Nong Van Hai

Main Article Content

Abstract

Abstract: Nostoc, widely distributed in cultivated soils, is one of the Cyanobacterial groups with filamental, hererocytous and autotrophic structures. This paper presents the results of isolating some Nostoc Cyanobacteria strains from selected cultivated soils in Nghe An province and evaluates their growth and nitrogen fixation for biomass production of bio-fertilizers. Five Nostoc strains were isolated, 3 of which looked blue-green (Nostoc calcicola HN9-1a, Nostoc linckia Cam-C1, Nostoc paludosum DT3-02) and the rest 2 looked brown (Nostoc ellipsosporum NH2X7, Nostoc gelatinosum TT3-05) in the white light. In BG-11 medium and neon light, the dried biomass productivity of the 3 blue-green strains (387 – 431 mg/L after 21 days of cultivation) was higher than that of the 2 brown strains’ (310 – 370 mg/L after 21 days of cultivation). These Nostoc strains are nitrogen fixing Cyanobacteria, which has been confirmed through detecting and sequencing their NifD gene and heterocyst formation. The results also show that the non-heterocyst Cyanobacterial strain, Pseudanabaena dictyothalla QV3-11, also isolated from the cultivated soil in Nghe An province, did not have the ability to fix nitrogen. The isolated nitrogen fixing Nostoc strains can be the subject of further research on bio-fertilizer production.


Keywords: Cyanobacteria, Nostoc, cultivated soil, growth, nitrogen fixation, NifD.


References


[1] Mai Van Chung, Nguyen Ba Hoanh, Nguyen Duc Dien, Nguyen Le Ai Vinh (2017) Nostoc calcicola extract improved the antioxidative reponse of soybean to cowpea aphid. Bot Stud 58: 55.
[2] Nguyễn Đức Diện, Nguyễn Lê Ái Vĩnh, Nguyễn Đình San, Võ Hành (2017) Ảnh hưởng của dịch nuôi chủng vi khuẩn lam Nostoc calcicola HN-91a đến sinh trưởng và năng suất giống lúa tám thơm thử nghiệm ở huyện Hưng Nguyên, tỉnh Nghệ An. Tạp chí Khoa học Trường Đại học Vinh 46 (3A): 13-19.
[3] Komárek J (2013) Band 19/3 – Cyanoprokaryota. Heterocytous Genera in Freshwater flora of Central Europe, edited by Büdel B, Gärtner G, Kriennitz L, Schagerl M. Heidelberg: Spektrum Akademischer Verlag.
[4] Neilan BA, Jacobs D, Goodman EA (1995), Genetic Diversity and Phylogeny of Toxic Cyanobacteria Determined by DNA Polymorphisms within the Phycocyanin Locus. Appl Environ Microbiol 61(11): 3875–3883.
[5] Lee RE (2008) Phycology. Cambridge University Press, New York.
[6] Roeselers G, Stal LJ, Loosdrecht MCM, Muyzer G (2007) Development of a PCR for the detection and identification of cyanobacteria nifD genes. J Microbiol Methods 70: 550-556.

Keywords: Từ khóa: Vi khuẩn lam, Nostoc, đất trồng, sinh trưởng, sự cố định nitrogen, gen NifD, Nghệ An.

References

[1] Mai Van Chung, Nguyen Ba Hoanh, Nguyen Duc Dien, Nguyen Le Ai Vinh (2017) Nostoc calcicola extract improved the antioxidative reponse of soybean to cowpea aphid. Bot Stud 58: 55.
[2] Nguyễn Đức Diện, Nguyễn Lê Ái Vĩnh, Nguyễn Đình San, Võ Hành (2017) Ảnh hưởng của dịch nuôi chủng vi khuẩn lam Nostoc calcicola HN-91a đến sinh trưởng và năng suất giống lúa tám thơm thử nghiệm ở huyện Hưng Nguyên, tỉnh Nghệ An. Tạp chí Khoa học Trường Đại học Vinh 46 (3A): 13-19.
[3] Komárek J (2013) Band 19/3 – Cyanoprokaryota. Heterocytous Genera in Freshwater flora of Central Europe, edited by Büdel B, Gärtner G, Kriennitz L, Schagerl M. Heidelberg: Spektrum Akademischer Verlag.
[4] Neilan BA, Jacobs D, Goodman EA (1995), Genetic Diversity and Phylogeny of Toxic Cyanobacteria Determined by DNA Polymorphisms within the Phycocyanin Locus. Appl Environ Microbiol 61(11): 3875–3883.
[5] Lee RE (2008) Phycology. Cambridge University Press, New York.
[6] Roeselers G, Stal LJ, Loosdrecht MCM, Muyzer G (2007) Development of a PCR for the detection and identification of cyanobacteria nifD genes. J Microbiol Methods 70: 550-556.