Microarray Data Analysis of as-induced Rice Roots by using Easygo and Mapman Softwares

Abstract

Rice is one of the most important crops in Asian countries such as China, Vietnam... Many recent reports indicate that the arsenic content in rice exceeds the threshold and affects human health. Studying of molecular mechanisms and finding the arsenic resistance genes in rice which is extremely important and urgent. In this study, we analyzed the transcriptional changes of arsenic-treated rice root cells during 24 hours by microarray technique. Results showed that a large number of the differentially expressed genes (720 genes). EasyGO and Mapman softwares are powerful tools in analyzing microarray data and classifying functional groups as well as the important metabolic pathways in the cell. Results of microarray analysis using EasyGO showed that 74 down-regulated genes related to cellular component, 200 up-regulated genes involved in catalytic activity, 93 up-regulated genes involved in biological processes as responding to environmental stress, and 64 detoxification-realted genes are increased expression such as cytochrome P450, Glutathione-S-transferase and UDP-Glycosyltransferase. Mapman's microarray analysis reaults also indicate that numerous of arsenic-tolerance genes of rice roots. These results support for searching indicated genes in the selection of As-tolerance rice varieties.
Keywords
Asen, EasyGO, Mapman, microarray, Oryza sativa L.

References

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