Culture Condition for High Biomass Production of Probiotic Yeast Saccharomyces Boulardii SB2 in Shake Flask and 30 Littre Scale

Abstract

Probiotic properties in vitroof the Saccharomyces boulardii SB2 yeast, was isolated from fruit at Cuc Phuong National Park were evaluated based on biological characteristics and tolerance in simulated intestinal conditions. In the present work, studies were carried out to improve cell growth kinetics to produce cell mass of this biotherapeutic yeast in shake flaskand 30-L bioreactor levels. In case of shake flask, the highest biomass was obtained under the culture conditions as YM medium, at 37^oC, pH 6, inoculum concentration of 5 %, with shaking for 32 h; the best sources of carbon and nitrogen were found to be glucose and mixture of peptone, yeast extract, malt extract; cell dry weight and cell density reached 2.43 g L^-1 and 10⁹ CFU/ml repetitively. During 30-L bioreactor cultivation, the maximal cell mass in agitation speed of 200 rpm, aeration rate of 1.2 v v^-1 min^-1; at 32 h of incubation, was 3.58 g L^-1 in pH controlled culture and 3.08 g L^-1 in uncontrolled pH. This is considered as the first step for cell mass production of this probiotic yeast in industrial scale
Keywords:
Saccharomyces boulardii SB2, probiotic, biomass, shake flask, 30-L bioreactor.

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