Factors Affecting the Output Signal of the Electrochemical Immunosensor Based on Chicken Egg Yolk Antibody for Detecting Newcastle Disease Virus

Tran Thi Luyen, Huynh Dang Chinh, Tran Quang Thinh, Mai Anh Tuan

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Published Dec 22, 2020
DOI: https://doi.org/10.25073/2588-1140/vnunst.5005

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LUYEN, Tran Thi et al. Factors Affecting the Output Signal of the Electrochemical Immunosensor Based on Chicken Egg Yolk Antibody for Detecting Newcastle Disease Virus. VNU Journal of Science: Natural Sciences and Technology, [S.l.], v. 36, n. 4, dec. 2020. ISSN 2588-1140. Available at: <https://js.vnu.edu.vn/NST/article/view/5005>. Date accessed: 22 dec. 2024. doi: https://doi.org/10.25073/2588-1140/vnunst.5005.
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Vol 36 No 4
Section
Original Articles

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Abstract

In this study, the electrochemical immunosensor for detecting Newcastle disease virus using the chicken egg yolk antibodies (IgY) was developed. The developed immunosensor was built into a three-electrode system consisting of two gold electrodes (a working electrode and a counter electrode) and a quasi-reference Ag/AgCl electrode. The IgY antibodies against Newcastle disease virus were immobilized on the working electrode (immunosensor) and the effect of the antibody concentration and virus incubation time on the output signal of the immunosensors was investigated. The investigation results show that when the antibody concentration was 60 µg/mL and the virus incubation time was 1 hour, the output signal of the immunosensors reached its maximum value (∆Ipeak = 0.1602). The detection limit of the sensors was 102 EID50/mL (EID50: 50% Empryo Infective Dose) at 25 oC. There was a good linear relationship between the ∆Ipeak and the logarithm of the virus Newcastle concentration in the range from 102 to 106 EID50/mL. The linear equation is ∆Ipeak = 0.0280logN – 0.00368 with the correlation coefficient of R2 = 0.9972.

Keywords: Electrochemical immunosensor, IgY antibody, Newcastle Disease virus (NDV), microchamber

References

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