Evaluation of the Toxicity of Dichlorodiphenyl-trichloroethane (DDT) to the Expression Levels of Three Genes p53, rarα1, wnt in the Embryos and Liver of Fish Medaka Oryzias latipes
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Abstract
Three genes p53, rarα1, wnt and liver of Medaka fish tissue were selected to study the toxicity of DDT to fish embryo (8 days old) and adult Medaka fish (3 months old). TEM and real-time PCR methods were used to examine changes in structure of liver tissue and genetic expression. The TEM results recorded a clear difference between the control and the experimental sample. Adult Medaka fish exposed to 1µg/L DDT for 24 hours has a distorted liver cell nucleus with many hollow lipid particles, and the sinus-shaped meshes are clustered, interrupted; lysosome is not intact with many degenerative bubbles. The results of real-time PCR analysis showed that DDT affects the development, different and proliferation of cells in the embryonic stage and gene expression in adult stage. All three genes p53, rarα1 and wnt in fish embryos changed strongly and tended to be inhibited when exposuring with 1700µg/L DDT, the values were recorded respectively 0.9; 4.9 and 5.4 times compared to the control sample (1 times). For adult fish, gene expression was lower than fish embryos with real-time PCR analysis values for the three genes p53, rarα1 and wnt respectively 0.9 and 0.5; 0.36 and 0.09; 0.53 and 0.09 times after exposure to 1500 and 1700 µg/L DDT. These results demonstrated that gene expression is dependent on the developmental stage of the cell as well as on the dose of the chemical exposed. The development, cell differentiation/proliferation in the embryonic and adulthood stages affected differently to the cell gene expression.
References
[2] D. Donaldson, Pesticide's Industry Sales and Usage 1998-1999 Market Estimates, US Environmental Protection Agency, Washington (DC): Available from: http: //www.epa.gov/oppbead/ pesticides/99 pestsales/market-estimates.pdf/, 2002 (accessed on: May 10th, 2021).
[3] F. Gao, J. Jia, X. Wand, Occurrence and Ordination of Dichlorodiphenyltrichloroethane and Hexachlorocyclohexane in Agricultural Soils from Guangzhou, China, Archives of Environmental Contamination and Toxicology, Vol. 54, 2008, pp. 155-166.
[4] IARC, DDT, Lindane, and 2,4-D”, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, World Health Organization, Vol. 113, 2018.
[5] R. M. Chinelo Chukwumalume, Polycyclic Aromatic Hydrocarbons (PAHs) and Organochlorine Pesticide Residues in Selected Marine Fish Species along the Coast of South Africa, Stellenbosch University Press, 2016.
[6] N. X. Tong, T. T. T. Huong, M. Huong, D. T. Thuy, N. H. T. Vy, The Impact of o, p`- DDT Pesticide Toxicity on the Growth of Medaka Fish Embryo Oryzias latipes, Vietnam Journal of Marine Science and Technology, Vol. 20, No. 1, 2020, pp. 73-81.
[7] T. D. Long, V. T. Thu, T. T. Thuy, Zebrafish and Medaka Fish as Models for Research on Human Diseases, The Conference of Youth Science and Technology in Medicine and Pharmacy 2th, 2015.
[8] K. Gormley, K. Teather, Developmental, Behavioral, and Reproductive Effects Experienced by Japanese Medaka (Oryzias latipes) in Response to Rhort-term Exposure to Endosulfan, Ecotoxicology and Environmental Safety, Vol. 54, No. 3, 2003, pp. 330-338.
[9] S. Bony, I. Gaillard, A. Devaux, Genotoxicity Assessment of Two Vineyard Pesticides in Zebrafish, International Journal of Environmental and Analytical Chemistry, Vol. 90, No. 3+6, 2010, pp. 421-428.
[10] F. Akcha, C. Spagnol, J. Rouxel, Genotoxicity of Diuron and Glyphosate in Oyster Spermatozoa and Embryos, Aquatic Toxicology, Vol. 106-107, 2012, pp. 104-113.
[11] R. Bate, T. Richar, DDT Saves Lives in Fight Against Malaria, Advancing Liberty-From the Economy to Ecology, 2006.
[12] A. Viarengo, D. Lowe, C. Bolognesi, E. Fabbri, A. Koehler, The Use of Biomarkers in Biomonitoring: A 2-tier Approach Assessing the Level of Pollutant-Induced Stress Syndrome in Sentinel Organisms, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology”, Vol. 146, No. 3, 2007, pp. 281-300.
[13] Y. Fuse, V. T. Nguyen, M. Kobayashi, Nrf2-Dependent Protection against Acute Sodium Arsenite Toxicity in Zebrafish, Toxicol Appl Pharmacol, Vol. 305,2016, pp. 136-142.
[14] I. Barjhoux, J. Cachot, P. Gonzalez, Transcriptional Responses and Embryotoxic Effects Induced by Pyrene and mMthylpyrene in Japanese Medaka (Oryzias latipes) Early Life Stages Exposed to Spiked Sediments, Environ Sci Pollut Res, Vol. 21, 2014, pp. 13850-13866.
[15] D. W. Klumpp, C. Humphrey, H. Huasheng, F. Tao, Toxic Contaminants and Their Biological Effects in Coastal Waters of Xiamen, China. II. Biomarkers and Embryo Malformation Rates as Indicators of Pollution Stress in Fish, Marine Pollution Bulletin, Vol. 44, 2002, pp. 752-760.
[16] T. Narahashi, Neurophysiological Effects of Insecticides, in Handbook of Pesticide Toxicology, 2010, pp. 799-816.
[17] G. N. Sreeya, R. Radha, C. N. Radhakrishnan, Studies on Acute Toxicity to Pesticide Stress in a Freshwater Fish Cirrhinus mrigala, International Journal of Fisheries and Aquatic Studies, Vol. 5, No. 4, 2017, pp. 355-358.
[18] Z. Zhang, J. Hu, Effects of p, p'-DDE Exposure on Gonadal Development and Gene Expression in Japanese Medaka (Oryzias latipes), J Environ Sci (China), Vol. 20, No. 3, 2008, pp. 347-352.
[19] J. Sun, C. Wang, H. Peng, G. Zheng, S. Zhang, J. Hu, p, p′ DDE Induces Gonadal Intersex in Japanese Medaka (Oryzias latipes) at Environmentally Relevant Concentrations: Comparison with o, p′ DDT, Environmental Science and Technology, Vol. 50, No. 1, 2015, pp. 462-469.
[20] C. V. David, S. W. Kullman, D. L. Howarth, R. C. Hardman, D. E. Hinton, Protective Response of the Ah Receptor to ANIT-Induced Biliary Epithelial Cell Toxicity in See-Through Medaka, Toxicological Sciences, Vol. 102, No. 2, 2008, pp. 262-277.
[21] T. Matsumoto, S. Terai, T. Oishi, S. Kuwashiro, K. Fujisawa, N. Yamamoto, Y. Fujita,
Y. Hamamoto, M. Furutani-Seiki, H. Nishina, I. Sakaida, Medaka as a Model for Human Nonalcoholic Steatohepatitis, Disease Models and Mechanisms, Vol. 3, 2010, pp. 431-440.
[22] M. Grinstein, H. L. Dingwall, L. D. O’Connor, A Distinct Transition from Cell Growth to Physiological Homeostasis in the Tendon, Elife, Vol. 8, 2019, pp. 48689.
[23] E. W. Surber, Effects of DDT on Fish, J. Wildl Manage, Vol. 10, 1946, pp. 183.
[24] J. L. Gerberding, Toxicological Profile for DDT, DDE and DDD, Agency for Toxic Substances and Disease Registry, USA Press, 2002.
[25] C. Haux, Å. Larsson, Effects of DDT on Blood Plasma Electrolytes in the Flounder, Platichthys Flesus L, in Hypotonic Brackish Water, Ambio, Vol. 8, 2002, pp. 171-173.
[26] M. Uchida, H. Nakamura, Y. Kagami, T. Kusano, K. Arizono, Estrogenic Effects of o, p'-DDT Exposure in Japanese Medaka (Oryzias latipes), The Journal of Toxicological Sciences, Vol. 35,
No. 4, 2010, pp. 605-608.
[27] B. P. Prasanth, Y. Shibata, R. Horiguchi, Exposure to Diethylstilbestrol During Embryonic and Larval Stages of Medaka Fish (Oryzias latipes) Leads to Sex Reversal in Genetic Males and Reduced Gonad Weight in Genetic Females, Endocrinology, Vol. 152, No. 2, 2011, pp. 707-717.