Nguyen Thi Thuy Van, Nguyen Dinh Viet, Duong Minh Lam

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Cordyceps sp. CPA14V was isolated from insect-fungi sample that was collected from Copia - Son La Nature Reserve. The strain was able to biosynthesize cyclooligomer depsipeptides (CODs). This study aimed to identify Cordyceps sp. CPA14V to species using DNA sequence analysis and to estimate the effects of carbon, nitrogen, pH of culture broth on its growth and COD producing capacity. The results showed that the studied strain was Cordyceps cateniannulata CPA14V. This is the first record of the species in Vietnam. Glucose, yeast extract and pH=8.0 were the most suitable for growth and CODs synthesis of C. cateniannulata CPA14V. This is also the first report of CODs produced by a C. cateniannulata.

Keywords: Cordyceps cateniannulata, cyclooligomer depsipeptides, carbon source, nitrogen source, pH.


[1] E. Gaumann, Ionophore Antibiotics Produced by the Fungus Fusarium Orthoceras var. Enniatum and other Fusaria, Experientia, Vol. 3, 1947, pp. 202-203.
[2] M. A. Abdalla, L. J. McGaw, Natural Cyclic Peptides as an Attractive Modality for Therapeutics: a Mini Review, Molecules, Vol. 23, No. 8, 2018, pp. 2080-2099,
[3] X. Wang, X. Gong, P. Li, D. Lai, L. Zhou, Structural Diversity and Biological Activities of Cyclic Depsipeptides from Fungi, Molecules, Vol. 23, No. 1, 2018, pp. 169-218,
[4] L. Zhang, O. E. Fasoyin, I. Molnár, Y. Xu, Secondary Metabolites from Hypocrealean Entomopathogenic Fungi: Novel Bioactive Compounds, Natural Product Reports, Vol. 37, No. 1, 2020, pp. 1181-1206,
[5] M. A. Fischbach, C. T. Walsh, Assembly-line Enzymology for Polyketide and Nonribosomal
Peptide Antibiotics: Logic, Machinery, and Mechanisms, Chemical Reviews, Vol. 106, No. 8, 2006, pp. 3468-3496,
[6] Q. Wang, L. Xu, Beauvericin, a Bioactive Compound Produced by Fungi: a Short Review, Molecules, Vol. 17, No. 3, 2012, pp. 2367-2377,
[7] H. S. Lee, H. H. Song, J. H. Ahn, C. G. Shin,
G. P. Lee, C. Lee, Statistical Optimization of Growth Medium for the Production of the Entomopathogenic and Phytotoxic Cyclic Depsipeptide Beauvericin from Fusarium Oxysporum KFCC 11363P, Journal of Microbiology and Biotechnology, Vol. 18, No. 1, 2008, pp. 138-144.
[8] L. J. Xu, Y. S. Liu, L. G. Zhou, J. Y. Wu, Optimization of a Liquid Medium for Beauvericin Production in Fusarium Redolens Dzf2 Mycelial Culture, Biotechnology and Bioprocess Engineering, Vol. 15, No. 3, 2010, pp. 460-466,
[9] H. Peeters, R. Zocher, N. Madry, H. Kleinkauf, Incorporation of Radioactive Precursors into Beauvericin Produced by Paecilomyces Fumoso-roseus, Phytochemistry, Vol. 22, No. 8, 1983, pp. 1719-1720,
[10] S. Das, B. Deb, DNA Barcoding of Fungi using Ribosomal ITS Marker for Genetic Diversity Analysis: A Review, Int. J. Pure Appl. Biosci, Vol. 3, No. 3, 2015, pp. 160-167.
[11] R. Lücking, M. C. Aime, B. Robbertse, A. N. Miller, T. Aoki, H. A. Ariyawansa, G. Cardinali, P. W. Crous, I. S Druzhinina, D. M. Geiser, Fungal Taxonomy and Sequence-based Nomenclature, Nature microbiology, Vol. 6, No. 5, 2021, pp. 540-548,
[12] T. X. Sinh, N. T. Thuy, Biodiversity of Entomopathogenic Fungi in Pu Mat National Park, Nghe An Province, Journal of Agriculture and Rural Development, Vol. 2, No. 1, 2013, pp. 28-35 (in Vietnamese).
[13] N. D. Viet, N. T. T. Van, T. X. Lam, D. M. Lam, Morphological and Molecular Characteristics of Isaria at Xuan Son National Park and Copia Nature Reserve, HNUE Journal of Science Natural Sciences, Vol. 66, No. 1, 2021, pp. 134-145, 10.18173/2354-1059.2021-0017 (in Vietnamese).
[14] N. N. Tuan, N. T. Thanh, T. D. Thang, Triterpenoid and Steroid from the Mycelium of Isaria Japonica Yadusa in Vietnam, Vinh University Journal of Science, Vol. 46, No. 3A, 2017, pp. 60-65 (in Vietnamese).
[15] J. Doyle, L. Doyle, A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue, Phytochemistry Bulletin, Vol. 19, No. 1, 1987, pp. 11-15.
[16] S. A. Rehner, G. J. Samuels, Taxonomy and Phylogeny of Gliocladium Analysed from Nuclear Large Subunit Ribosomal DNA Sequences, Mycological Research, Vol. 98, No. 6, 1994, pp. 625-634,
[17] R. Vilgalys, M. Hester, Rapid Genetic Identification and Mapping of Enzymatically Amplified Ribosomal DNA from Several Cryptococcus Species, Journal of Bacteriology, Vol. 172, No. 8, 1990, pp. 4238-4246,
[18] L. A. Castlebury, A. Y. Rossman, G. H. Sung, A. S. Hyten, J. W. Spatafora, Multigene Phylogeny Reveals New Lineage for Stachybotrys Chartarum, the Indoor Air Fungus, Mycological Research, Vol. 108, No. 8, 2004, pp. 864-872,
[19] T. Hall, BioEdit: A User-friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT, Nucleic Acids Symp, Ser, 1999, pp. 95-98.
[20] J. D. Thompson, T. J. Gibson, F. Plewniak, F. Jeanmougiz, D. G. Higgins, The CLUSTAL_X Windows Iterface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools, Nucleic Acids Research, Vol. 25, No. 24, 1997, pp. 4876-4882,
[21] S. Kumar, G. Stecher, M. Li, C. Knyaz, K. Tamura, MEGA X: Molecular Evolutionary Genetics Analysis Across Computing Platforms, Molecular Biology and Evolution, Vol. 35, No. 6, 2018, pp. 1547,
[22] J. Smedsgaard, Micro-scale Extraction Procedure for Standardized Screening of Fungal Metabolite Production in Cultures, Journal of Chromatography A, Vol. 760, No. 2, 1997, pp. 264-270,
[23] J. J. Luangsa-Ard, P. Berkaew, R. Ridkaew, N. L. Hywel-Jones, M. Isaka, A Beauvericin Hot Spot in the Genus Isaria, Mycological Research, Vol. 113, No. 12, 2009, pp. 1389-1395,
[24] A. Logrieco, A. Moretti, G. Castella, M. Kostecki, P. Golinski, A. Ritieni, J. Chelkowski, Beauvericin Production by Fusarium Species, Appl, Environ, Microbiol, Vol. 64, No. 8, 1998, pp. 3084-3088,