Induction of Apoptosis and Inhibition of Breast Cancer Cell Growth and Multicellular Tumor Spheroids by Paclitaxel Combined Curcumin-loaded PLA-TPGS-based Nanoparticles
Main Article Content
Abstract
Paclitaxel and curcumin have been reported as anti-cancer compounds. Here, we presented a novel combination of paclitaxel and curcumin-loaded PLA-TPGS (PTX-Cur/PLA-TPGS) nanoparticles prepared by a modified solvent extraction/evaporation technique. These nanoparticles were well distributed and stable in water. This combination of paclitaxel and curcumin gave a higher efficiency of both drugs in cytotoxicity, induced apoptosis, and effect on cell cycles of KPL4 cell line in comparison with the use of paclitaxel or curcumin alone or even a normal mixture of these two compounds. Furthermore, PTX-Cur/PLA-TPGS nanoparticles exhibited a powerful ability in preventing MCF7 spheroids growth. Interestingly, curcumin also functioned as both a drug and a label. Based on the autofluorescence of curcumin, the absorption of PTX-Cur/PLA-TPGS nanoparticles into MCF7 spheroids could be followed and calculated. These results suggest that the nanoparticle-combination may provide a promising multifunctional delivery system for anti-cancer drugs.
Keywords:
Paclitaxel, curcumin, PLA-TPGS nanoparticles, breast cancer, apoptosis, multicellular tumor spheroid.
References
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[23] M. V. Blagosklonny, Mitotic Arrest and Cell Fate: Why and How Mitotic Inhibition of Transcription Drives Mutually Exclusive Events, Cell Cycle, Vol. 6, No. 1, 2007, pp.70-4, https://doi.org/10.4161/cc.6.1.3682.
[24] L. A. K. Schughart, J. P. Freyer, F. Hofstaedter, R. Ebner, The Use of 3-D Cultures for High-throughput Screening: the Multicellular Spheroid Model, J. Biomol, Screen, 2004, pp. 273-85, https://doi.org/10.1177/1087057104265040.
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