Nguyen Dac Tu, Tran Phan Anh, Ha Phuong Thu, Nguyen Hoai Nam, Nguyen Xuan Phuc, Hoang Thi My Nhung

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Paclitaxel and curcumin have been reported as anti-cancer compounds. Here, we presented a novel combination of paclitaxel and curcumin-loaded PLA-TPGS (PTX-Cur/PLA-TPGS) nanoparticles prepared by a modified solvent extraction/evaporation technique. These nanoparticles were well distributed and stable in water. This combination of paclitaxel and curcumin gave a higher efficiency of both drugs in cytotoxicity, induced apoptosis, and effect on cell cycles of KPL4 cell line in comparison with the use of paclitaxel or curcumin alone or even a normal mixture of these two compounds. Furthermore, PTX-Cur/PLA-TPGS nanoparticles exhibited a powerful ability in preventing MCF7 spheroids growth. Interestingly, curcumin also functioned as both a drug and a label. Based on the autofluorescence of curcumin, the absorption of PTX-Cur/PLA-TPGS nanoparticles into MCF7 spheroids could be followed and calculated. These results suggest that the nanoparticle-combination may provide a promising multifunctional delivery system for anti-cancer drugs.

Keywords: Paclitaxel, curcumin, PLA-TPGS nanoparticles, breast cancer, apoptosis, multicellular tumor spheroid.


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