Pham Thi Bich, Vu Thi Thu

Main Article Content


This study was carried out to evaluate the effect of Cobalt chloride (CoCl2) on cardiac mitochondrial function in an in vitro model. In the study, H9C2 cardiomyocytes were cultured in a medium containing different concentrations of CoCl2. Cell viability, cardiolipin content, mitochondrial function, and mitochondrial oxidative stress were assessed by using Cell Counting Kit-8 and suitable fluorescence kits. The obtained data show that CoCl2 (200÷400 µM) induced cell death and decreased mitochondrial function of H9C2 cardiomyocytes. Particularly, CoCl2 at the dose of 300 µM significantly altered the values of mitochondrial membrane potential, H2O2 and O2- to 63.79±2.15%, 145.81±5.83% and 143.10±3.07% (of 100% control), respectively. Altogether, CoCl2 strongly induced cardiomyocyte death via altering mitochondrial function in a dose-dependent manner.

Keywords: H9C2, mitochondria, cell counting kit-8.


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