Evaluation of Microbial Contamination Rates in Umbilical Cord Blood Collection and Processing for Biobanking in Vietnam
Main Article Content
Abstract
The umbilical cord blood (CB) has recently been considered an abundant source of hematopoietic stem cells (HSCs) for transplantation compared to bone marrow. However, the collection and processing of CB have a high risk of microbial contamination. Hence, the procedures to collect and process the UCB are carefully considered. This study evaluates the microbial contamination rate to find the frequency and distribution of bacterial organisms among CB sampling and processing in Vietnam. In addition, this study correlates with contamination rates between the delivery method, cesarean section, and vaginal delivery. The results create best practices to avoid a high level of contamination of UCB during collection and processing for biobanking.
Keywords:
Microbial contamination, Umbilical cord blood, Biobanking, Umbilical cord blood bank.
References
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[3] E. Gluckman, H. E. Broxmeyer, A. D. Auerbach, H. S. Friedman, G. W. Douglas, A. Devergie et al., Hematopoietic Reconstitution in a Patient With Fanconi’s Anemia by Means of Umbilical-cord Blood from an HLA-identical Sibling, N. Engl, J. Med, Vol. 321, 1989, pp. 1174-8.
[4] S. Berglund, I. Magalhaes, A. Gaballa, B. Vanherberghen, Advances in Umbilical Cord Blood Cell Therapy: the Present and the Future, Expert Opin Biol Ther, Vol. 17, 2017, pp. 6919, https://doi.org/10.1080/14712598.2017.1316713.
[5] S. H. Mousavi, S. Abroun, M. Soleimani, S. J. Mowla, Expansion of Human Cord Blood Hematopoietic Stem/progenitor Cells in Three-dimensional Nanoscaffold Coated with Fibronectin, Int, J. Hematol Stem Cell Res, Vlo. 9, 2015, pp. 72-9.
[6] S. H. Mousavi, M. Zarrabi, S. Abroun, M. Ahmadipanah, B. Abbaspanah, Umbilical Cord Blood Quality and Quantity: Collection up to Transplantation, Asian, J. Transfus Sci, Vol. 13, 2019, pp. 79-89, https://doi.org/10.4103/ajts.AJTS.
[7] K. Ballen, F. Verter, J. Kurtzberg, Umbilical Cord Blood Donation: Public or Private? Bone Marrow Transplant, Vol. 50, 2015, pp. 1271-8, https://doi.org/10.1038/bmt.2015.124.
[8] P. Clark, A. Trickett, M. Chimenti, D. Stark, Optimization of Microbial Screening for Cord Blood, Transfusion, Vol. 54, 2014, pp. 550-9, https://doi.org/10.1111/trf.12352.
[9] P. Clark, A. Trickett, S. Saffo, D. Stark, Effects of Cryopreservation on Microbial - Contaminated Cord Blood, Transfusion, Vol. 54, 2014, pp. 532-40, https://doi.org/10.1111/trf.12323.
[10] S. R. Arcos, Y. Kou, L. Yang, H. Perkins, M. Taha, M. Halpenny et al., Validation of Sterility Testing of Cord Blood: Challenges and Results, Transfusion, Vol. 55, 2015, pp. 1985-92, https://doi.org/10.1111/trf.13050.
[11] P. Clark, A. Trickett, D. Stack, M. Vowel, Factors Affecting Microbial Contamination Rate of Cord Blood, Transfusion, Vol. 52, 2012, pp. 1770-7, https://doi.org/10.1111/j.1537-2995.2011.03507.x.
[12] M. Li, D. Lim, K. F. Tang, S. Lim, K. Boey, Microbial Contamination in Umbilical Cord Blood: A Comparison Before and After Cryopreservation, Stem Cells Transl Med, Vol. 7, 2018, pp. S2, https://doi.org/10.1002/sctm.12354.
[13] E. M. Areman, K. Loper, Cellular Therapy: principles, Methods, and Regulations, AABB, 2009.
[14] S. Armitage, R. Warwick, D. Fehily, C. Navarrete, M. Contreras, Cord Blood Banking in London: the First 1000 Collections, Bone Marrow Transplant, Vol. 24, 1999, pp. 139-45.
[15] U. Elchalal, S. J. Fasouliotis, D. Shtockheim, C. Brautbar, Postpartum Umbilical Cord Blood Collection for Transplantation: A Comparison of Three Methods, Am, J. Obstet Gynecol, Vol. 182, 2000, pp. 227-32.
[16] N. M. Reboredo, D. Diaz, A. Castro, R. Villaescusa, R. Collection, Processing and Cryopreservation of Umbilical Cord Blood for Unrelated Transplantation, Bone Marrow Transplant, Vol. 26, 2000, pp. 1263-70.
[17] A. Honohan, H. Othuis, A. Bernards, J. M. van Beckhoven, A. Brand, Microbial Contamination of Cord Blood Stem Cells, Vox Sang, Vol. 82, 2002, pp. 32-8.
[18] S. Chen, Y. Zheng, S. Yang, K. Yang, M. Shyr, Y. Ho, Microbial Contamination of the Tzu-Chi Cord Blood Bank from 2005 to 2006, Acta Paediatr Taiwan, Vol. 49, 2008, pp. 9-13.
[19] J. M. B. López, J. N. Silva, J. I. Casta, J. R. Medina, Molecular Characterization of Microbial Contaminants Isolated from Umbilical Cord Blood, Brazilian, J. Infect Dis, Vol. 9, 2015, pp. 571-7. https://doi.org/10.1016/j.bjid.2015.07.005.
[20] H. Rafii, F. Bernaudin, H. Rouard, V. Vanneaux, A. Ruggeri, M. Cavazzana et al., Family Cord Blood Banking for Sickle Cell Disease: a Twenty-year Experience in Two Dedicated Public Cord Blood Banks, Haematologica, Vol. 102, 2017, PP. 976-83, https://doi.org/10.3324/haematol.2016.163055.
[21] D. Vanegas, T. Lady, C. Galindo C, L. Franco, G. Salguero, B. Camachoet al., A New Strategy for Umbilical Cord Blood Collection Developed at the First Colombian Public Cord Blood Bank Increases Total Nucleated Cell Content, Transfusion, Vol. 57, 2017, pp. 2225-33, https://doi.org/10.1111/trf.14190.
[22] M. Ibrahim, S. A. L. Hajali, M. Abdelmeguid,S. Aswad, Contamination Rates by Delivery Method of Human Umbilical Cord Blood Samples in the United Arab Emirates and Gulf Co-operation Council Countries, Int, J. Stem Cell Res Ther, Vol. 7, 2020, pp. 1-4, https://doi.org/10.23937/2469-570X/1410067.