Characterization of Adipose‐derived Stem Cells from Breast Cancer Cell Transplanted Mice
Main Article Content
Abstract
Mesenchymal stem cells (MCSs) from adipose tissue are reported to have a pronounced impact on tumor growth or suppression. However, so far, how the cancer cells affect the stem cells in vivo conditions has not been clarified. In this study, we evaluated the impact of breast cancer cells on the characteristics of adipose-derived stem cells (ADSCs) under in vivo conditions. ADSCs were obtained from Balb/c mice in three consecutive weeks after transplantation with 1×106 MCF7 cells. The results showed that the amount of ADSCs per one gram fat reduced over time, but that reduction was not significantly different. In fact, isolated ADSCs presenting specific MSC surface markers increased at the second passage, compared to the time of isolation. Particularly, they were highly positive for CD90 (a MSC indicator) and negative for CD45 (a hematopoietic marker). Moreover, in in vitro culture, ADSCs were successfully differentiated into adipocytes, which were detected by Oil Red O staining. This present experiment gives initial assessments of ADSC characteristics; however, further investigations are necessary to fully evaluate the differences in stem cell potency and immunophenotype caused by cancer cell transplantation.
References
[2] N. Yamamoto, H. Akamatsu, S. Hasegawa, T. Yamada, S. Nakata, M. Ohkuma, E. Miyachi, T. Marunouchi, K. Matsunaga, Isolation of Multipotent Stem Cells from Mouse Adipose Tissue, Journal of Dermatological Science,
Vol. 48, No. 1, 2007, pp. 43-52, https://doi.org/10.1016/j.jdermsci. 2007. 05.015.
[3] N. V. Andreeva, A. Dalina, A. V. Belyavsky, Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue, Bio-protocol, Vol. 7, No. 16, 2017, pp. e2516, https://doi.org/10.21769/ BioProtoc.2516.
[4] Y. Chen, Y. He, X. Wang, F. Lu, J. Gao, Adipose‑derived Mesenchymal Stem Cells Exhibit Tumor Tropism and Promote Tumorsphere Formation of Breast Cancer Cells, Oncology Reports, Vol. 41, 2019, pp. 2126-2136,
https://doi.org/doi: 10.3892/or.2019.7018.
[5] WHO, Breast Cancer, https://www.who.int/news-room/fact-sheets/detail /breast-cancer.
[6] F. Rey, E. Lesma, D. Massihnia, E. Ciusani, S. Nava, C. Vasco, G. A. Haj, G. Ghilardi, E. Opocher, A. Gorio, S. Carelli, A. M. D. Giulio, Adipose-derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities, Hindawi Stem Cells International, Vol. 2019, pp. 15, https://doi.org/10.1155/2019/ 1480314.
[7] Committee for the Update of the Guide for the Care and Use of Laboratory Animals, National Research Council Guide for the Care and Use of Laboratory Animals, Eighth Edition, National Academies Press, USA, 2010.
[8] R. C. Oropeza, K. V. Santillan, C. D. Gastelum, J. M. Zajgla, C. Zampedri, E. F. Osorio, A. C. Oropeza, R. K. V. Santillan, C. D. Gastelum, J. M. Zajgla, C. Zampedri, E. F. Osorio, A. R. González, L. A. Pizano, V. Maldonado, Adipose-derived Mesenchymal Stem Cells Promote the Malignant Phenotype of Cervical Cancer, Science Report, Vol. 10, 2020, pp. 14205, https://doi.org/10.1038/ s41598-020-69907-x.
[9] J. Zhang, Y. Liu, W. Yin, X. Hu, Adipose-derived Stromal Cells in Regulation of Hematopoiesis, Cellular & Molecular Biology Letters, Vol. 25, 2020, pp. 16, https://doi.org/10.1186/s11658-020-00209-w.
[10] P. C. Baer, H. Geiger, Adipose-derived Mesenchymal Stromal/stem Cells: Tissue Localization, Characterization, and Heterogeneity, Stem Cells International, Vol. 2012, 2012, pp. 812693, https://doi.org/10.1155/2012/812693.
[11] N. T. Thuy, T. N. Vu, C. T. C. Ha, Isolation and Culture of Adipose - derived Mesenchymal Stem Cells from Mice (Swiss) Adipose Tissue, Can Tho Journal of Medicine and Pharmacy, Vol. 6, No. 2, 2020, pp. 372-379 (in Vietnamese).
[12] A. C. Luna, M. E. Madeira, T. O. Conceição, J. A. Moreira, R. A. Laiso, D. A. Maria, Characterization of Adipose-derived Stem Cells of Anatomical Region from Mice, BMC Research Notes, Vol. 7, 2014, pp. 552, https://doi.org/10.1186/1756-0500-7-552.
[13] N. T. Phuong, N. H. Hung, A Simple Non-enzymatic Protocol for Isolation and Culture of Mesenchymal Stem Cells from Adipose Tissues, Journal of Science and Technology, Vol. 2, 2018, pp. 12-16.
[14] M. F. Taha, V. Hedayati, Isolation, Identification and Multipotential Differentiation of Mouse Sdipose Tissue-derived Stem Cells, Tissue Cell, Vol. 42, No. 4, 2010, pp. 211-216, https://doi.org/10.1016/j.tice. 2010.04.003.
[15] J. R. Maddox, X. Liao, F. Li, C. Niyibizi, Effects of Culturing on the Stability of the Putative Murine Adipose Derived Stem Cells Markers, The Open Stem Cell Journal, Vol. 1, 2009, pp. 54-61, https://doi.org/10.2174/1876893800901010054.