Investigation of Botulinum Neurotoxin Types from Clostridium botulinum Causing A Recent Outbreak in Vietnam

La Thi Huong Huyen, Nguyen Thuy Linh, Nguyen Thanh Trung, Pham Yen

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Published Dec 7, 2021
DOI: https://doi.org/10.25073/2588-1140/vnunst.5332

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HUYEN, La Thi Huong et al. Investigation of Botulinum Neurotoxin Types from Clostridium botulinum Causing A Recent Outbreak in Vietnam. VNU Journal of Science: Natural Sciences and Technology, [S.l.], v. 37, n. 4, dec. 2021. ISSN 2588-1140. Available at: <https://js.vnu.edu.vn/NST/article/view/5332>. Date accessed: 26 dec. 2024. doi: https://doi.org/10.25073/2588-1140/vnunst.5332.
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Issue
Vol 37 No 4
Section
Original Articles

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Abstract

Clostridium botulinum is one of the causes of undiagnosed sudden deaths in humans due to the lethal botulinum neurotoxins (BoNTs). Foodborne botulism rarely occurs in developed countries because of being closely monitored, in opposite to developing countries including Vietnam. In the August 2020 food poisoning outbreak in Vietnam, presence of Clostridium botulinum and BoNTs was identified by culture and mouse bioassay, however, information regarding the possible toxin types was unclear. To examine the types of toxin, we designed primers for specific amplification of gene regions encoding the light chain (LC) domains for both BoNT/A and BoNT/B. After optimization, the expected PCR products were sent for sequencing. The results showed that the sequence of gene encoding BoNT/A LC was 99.2% identical to the CB-27 strain. The sequence of gene encoding BoNT/B LC was approximately 98.8% identical to reference strains. Additionally, we analyzed the sequences of the inferred proteins and identified a substitution that resulted in an early stop codon as previously found in a defective form of BoNT/B. Collectively, we provided the first evidence for C. botulinum strain possessing A(b) type in this studied outbreak. Further enzyme activity and neutralization assays are necessary to validate this preliminary toxin typing.

Keywords: Clostridium botulinum, BoNT neurotoxin, bont genes, light chain.

References

1. D. Lonati, et al., Foodborne Botulism: Clinical Diagnosis and Medical Treatment, Toxins (Basel), 12(8):509, 2020, http://doi.org/10.3390/toxins12080509.
2. S. Fleck- Derderian, M. Shankar, A. K. Rao, The Epidemiology of Foodborne Botulism Outbreaks: A Systematic Review, Clinical Infectious Disease, 66, 2018, pp. 73-81, http://doi.org/10.1093/cid/cix846.
3. C. Montecucco, M. B. Rasotto, On botulinum neurotoxin variability, MBio, 6(1), 2015, pp. e02131-14, http://doi.org/10.1128/mBio.02131-14.
4. K. R. Aoki, B. Guyer, Botulinum toxin type A and other botulinum toxin serotypes: a comparative review of biochemical and pharmacological actions, European Journal of Neurology, 8(5), 2001, http://doi.org/10.1046/j.1468-1331.2001.00035.
5. S. Pellett, et al., The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A Subtypes, mBio, 9(2), 2018, http://doi.org/10.1128/mBio.00089-18.
6. R. M. Benoit, Botulinum Neurotoxin Diversity from a Gene-Centered View, Toxins (Basel), 10(8), 2018, http://doi.org/10.3390/toxins10080310.
7. M. W. Peck, T. J. Smith, Historical perspectives and guidelines for botulinum neurotoxin subtype nomenclature, Toxin 9(38), 2017, http://doi.org/10.3390/toxins9010038.
8. K. K. Hill, et al., Recombination and insertion events involving the botulinum neurotoxin complex genes in Clostridium botulinum types A, B, E and F and Clostridium butyricum type E strains, BMC Biol, 7:66, 2009, http://doi.org/10.1186/1741-7007-7-66.
9. G. Kathleen, M. Kristin, Foodborne Infections and Intoxications, Food Science and Technology, Chapter 27, 2013, pp. 371-387.
10. C. S. Bever, et al., Development and Characterization of Monoclonal Antibodies to Botulinum Neurotoxin Type E, Toxins (Basel), 11(7):407, 2019, http://doi.org/10.3390/toxins11070407.
11. W. Piyada, K. Tomoko, S. Ahmed, et al., Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010, PLoS ONE, 9(1), 2014, e77792, http://doi.org/10.1371/journal.pone.0077792.
12. S. Zhang, Y. Wang, et al., Multilocus outbreak of foodborne botulism linked to contaminated sausage in Hebei province, China, Clin Infect Dis, Vol. 51, No. 3, 2010, pp. 322-325.
13. J. H. Jeon, G. Rhie, et al., Genetic Characterization of Clostridium botulinum Isolated from the First Case of Infant Botulism in Korea, Ann Lab Med, Vol. 41, No. 5, 2021, pp. 489-492.
14. M. H. David, J. B. James, An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis, Systematic Biology, Vol. 42, No. 2, 1993, pp. 182-192.
15. R. K. Suzanne, B. Jakub, De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics, Anal Bioanal Chem, Vol. 403, No. 1, 2012, pp. 215-226.
16. A. P. Gardner, J. T. Barbieri, Light Chain Diversity among the Botulinum Neurotoxins, Toxins (Basel), 10(7):268, 2018, http://doi.org/10.3390/toxins10070268.
17. R. A. Hutson, Genetic characterization of Clostridium botulinum type A containing silent type B neurotoxin gene sequences, Journal of Biological Chemistry, Vol. 271, No. 18, 1996, pp. 10786-10792.