Pham Anh Thuy Duong, Nguyen Thu Trang, Nguyen Thuy Ngan, Vo Thi Thuong Lan

Main Article Content


Epigenetic alterations play a main role in the initiation and progression of lung cancer.  CpG methylation in the promoter of the Short Stature Homeobox 2 (SHOX2) gene has been evaluated and validated at different stages of this malignant disease. Several approaches for measuring DNA methylation have been established, including quantitative methylation-specific PCR (qMSP). This is a simple, fast, and cost-effective technique that can be easily applied to clinical practice. In this study, formalin-fixed, paraffin-embedded (FFPE) tissue samples were collected from 30 lung cancer patients and 30 patients suffering from non-cancerous pulmonary diseases.  The methylation level of SHOX2 was evaluated in two CpG-riched regions by using qMSP and one of them could be conferred as a potential biomarker to lung cancer.

Keywords: DNA methylation, SHOX2, lung cancer, quantitative methylation-specific PCR (qMSP)


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