Evaluation the Ability to Isolate and Culture Stem Cells from Frozen Human Umbilical Cord Tissue
Main Article Content
Abstract
Umbilical cord-derived mesenchymal stem cells have been determined to be effective in cell therapy and regenerative medicine. Cell cryopreservation is widely used for long term preservation. However, cell cryopreservation is costly and labor consume. Therefore, cryopreservation of human umbilical cord (HUC) tissue has become an alternative solution to reduce the risks of disease and open up opportunities for later treatment for families in recent years. In this study, we evaluated the ability to isolate and the characteristic of mesenchymal stem cells (MSCs) derived from fresh and frozen human umbilical cord tissue. The results showed that the HUCMSCs were isolated successfully from both fresh and frozen HUC tissues. The expression of MSCs cell surface marker CD73, CD90, CD105 in group 1 were higher than those in group 2, CD73 (99,42 vs 95.2%); CD90 (99.74 vs 96.6%); CD105 (99.64 vs 96.15%), respectively. However, they are similar in morphology and osteogenic and adipose differentiated ability. The results of the study would be useful for stem cell research, cell therapy and regenerative medicine.
Keywords:
Cell therapy, cryopreservation, mesenchymal stem cell, human umbilical cord tissue.
References
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[7] T. K. Do, N. Van Hanh, T. N. Nguyen, N. Viet Linh, D. M. Pham, N. T. N. Nguyen, T. T. H. Tran, T. T. H. Ngo, H. H. Chu, Efficient Isolation and Long-term Red Fluorescent Nanodia-mond Labeling of Umbilical Cord Mesenchymal Stem Cells for the Effective Differentiation into Hepatocyte-like Cells, Braz, Arch, Biol, Technol, Vol. 63, 2020, pp. e20200082, https://doi.org/ 10.1590/1678-4324-202020008.
[8] A. De Rosa, F. De Francesco, V. Tirino, G. A. Ferraro, V. Desiderio, F. Paino, G. Pirozzi, F. D’Andrea, G. Papaccio, A New Method for Cryopreserving Adiposederived Stem Cells: an Attractive and Suitable Large-scale and Long-term Cell Banking Technology, Tissue Eng Part C Methods, Vol. 15, No. 4, 2009, pp. 659-667, https://doi.org/10.1089/ ten.TEC.2008.0674.
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https://doi.org/10.1038/ncb2719.
[12] G. Cai, B. Lai, H. Hong, P. Lin, W. Chen, Z. Zhu, H. Chen, Effects of Cryopreservation on Excretory Function, Cellular Adhesion Molecules and Vessel Lumen Formation in Human Umbilical Vein Endothelial Cells, Mol Med Rep, Vol. 16, No. 1, 2017, pp.547-552, https://doi.org/10.3892/mmr.2017.6664.
[13] X. Fu, B. Xu, J. Jiang, X. Du, X. Yu, Y. Yan, S. Li, B. M. Inglis, H. Ma, H. Wang, X. Pei, W. Si, Effects of Cryopreservation and Long-term Culture on Biological Characteristics and Proteomic Profiles of Human Umbilical Cord-derived Mesenchymal Stem Cells, Clin Proteomics, Vol. 17, 2020, pp. 15, https://doi.org/10.1186/s12014-020-09279-6.
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